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一般說明
Apolipoprotein is a polymorphic plasma protein that exists as three isoforms: ApoE4, ApoE3, and ApoE2, which differ in the combination of cysteine and arginine residues located at position 122 and 158. The 35 kDa ApoE4 isoform contains arginine at both locations, and is produced in the liver and brain, although it is widely expressed in other tissues, such as the lung, spleen, and ovary. Like other isoforms, ApoE4 is a ligand for low density lipoprotein receptors (LDL) and plays a critical role in the transport and metabolism of lipids. In addition to its role in maintaining cardiovascular function, ApoE4 is also critically involved in neuronal plasticity, synaptogenesis, aging, and neurodegenerative disorders. Mice deficient in ApoE4 show age-dependent abnormalities in synaptic structure and impaired long term potentiation. Additionally, ApoE4 has a well-documented role in the pathology of Alzheimer’s disease.
特異性
This antibody recognizes ApoE4 but not ApoE3 or ApoE2.
免疫原
KLH-conjugated linear peptide corresponding to human ApoE4.
應用
Immunohistochemistry Analysis: A 1:2,000-4,000 dilution from a representative lot detected ApoE4 in human Alzheimer′s diseased brain and human cerebellum tissues.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect ApoE4 in CHO cells secreting ApoE4. (Image courtesy of Dr. Egon Ogris, Medical University of Vienna)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect ApoE4 in CHO cells secreting ApoE4. (Image courtesy of Dr. Egon Ogris, Medical University of Vienna)
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
This Anti--Apolipoprotein E4 antibody is validated for use in IP, WB, IH for the detection of ApoE4.
品質
Evaluated by Western Blot in recombinant ApoE4 protein.
Western Blot Analysis: A 1:100,000 dilution detected ApoE4 in 10 µg of recombinant ApoE4 protein.
Western Blot Analysis: A 1:100,000 dilution detected ApoE4 in 10 µg of recombinant ApoE4 protein.
標靶描述
~35 kDa observed
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
分析報告
Control
Recombinant Apolipoprotein E protein
Recombinant Apolipoprotein E protein
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Hee-Young Sohn et al.
Scientific reports, 11(1), 17604-17604 (2021-09-04)
Apolipoprotein E (ApoE) plays multiple roles in lipid transport, neuronal signaling, glucose metabolism, mitochondrial function, and inflammation in the brain. It is also associated with neurodegenerative diseases, and its influence differs depending on the isoform. In particular, the ε4 allele
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