MABF3208

Anti-HPV11 Antibody, clone H11.H3

• 别名: HPV-11, Major capsid protein L1
• 分類程式碼代碼: 12352203

属性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: H11.H3, monoclonal
• 分子量: calculated mol wt 55.8 kDa; observed mol wt ~N/A kDa
• 純化經由: using protein G
• 物種活性: virus
• 包裝: antibody small pack of 100
• 技術: ELISA: suitable; neutralization: suitable
• 同型: IgG2bκ
• Protein ID登錄號: N/A
• UniProt登錄號: P04012
• 儲存溫度: -10 to -25°C

说明

特異性

Clone H11.H3 is a mouse monoclonal antibody that detects HPV11. It targets an epitope within the Major capsid protein L1.

免疫原

Intact HPV-11 virions obtained from morphologically transformed human foreskin xenografts grown subrenally in athymic mice.

應用

Quality Control Testing

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b, kappa.

Tested Applications

Enzyme Immunoassay Analysis: A representative lot of this antibody detected HPV11 protein in ELISA application.

Neutralizing: A representative lot of this antibody neutralized HPV11 in neutralizing application (Christensen, N.D., et al. (1994). Virology. 205(1):329-35; Christensen, N.D., et al. (1996). Virology. 224(2):477-86).

ELISA Analysis: A representative lot of this antibody detected HPV11 protein in ELISA application (Christensen, N.D., et al. (1990). J Virol. 64(11):5678-81; Christensen, N.D., et al. (1994). Virology. 205(1):329-35 ; Christensen, N.D., et al. (1996). Virology. 224(2):477-86; Panicker, G., et al. (2021). J Immunol Methods. 498:113136; Jerajani, K., et al. (2022). J Pharm Sci. 111(11):2983-2997).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Major capsid protein L1 (UniProt: P04012) is encoded by the L1 gene in Human papillomavirus 11. The Major Capsid Protein L1 of Human papillomavirus is the primary structural protein responsible for the formation of the viral capsid, that encapsulates the genomic DNA together with Minor Capsid Protein L2. The viral capsid is composed of 360 copies of the L1 protein forming 72 pentamers, that assemble into the icosahedral capsid structure, while the L2 protein localizes along the inner surface of the virion and plays a role in capsid stabilization through interaction with the Major Capsid Protein L1. The L1 protein facilitates the attachment of the virus to the host cell by interacting with heparan sulfate proteoglycans on the cell surface, a critical step for infection. Recombinantly expressed Major Capsid Protein L1 can spontaneously assemble into Virus Like-Particles (VLPs) and were used for the development of neutralizing antibodies that show high degree of Human papillomavirus type-specificity. Based on their oncogenic potential, Human papillomaviruses (HPVs) can be classified as low-risk HPV types, (e.g.: HPV6 and HPV11, the most common serotypes) which do not cause cancer but can lead to skin and genital warts, and high-risk HPV types (e.g.: HPV 16, 18, 31, 33, 35, 45, 52, 58), that are responsible for HPV related cancers. Vaccines targeting the L1 protein, have been developed and are effective in preventing infection with the most oncogenic HPV types by inducing a robust antibody response. (Ref.: Hareza, D. A., (2022) Int. J. Mol. Sci., 23, 1818.; Christensen, N.D., et al. (1990). J Virol. 64:11, 5678-81; Christensen, N.D., et al. (1996). Virology. 224(2):477-86; Brown, M.J., et al. (2014). Clin Vaccine Immunol. 21:4, 587-93)

外觀

Purified mouse monoclonal antibody IgG2b in PBS without preservatives.

重構

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable