MABC519

Anti-Trop2, clone 2G10, Azide Free Antibody

clone 2G10, from mouse

• 别名: Tumor-associated calcium signal transducer 2, Cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 2G10, monoclonal
• 物種活性: human
• 技術: flow cytometry: suitable; immunocytochemistry: suitable; inhibition assay: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_002344
• UniProt登錄號: P09758
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... TACSTD2(4070)

说明

一般說明

The protein known as Trop-2, or also known as cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1 or Pancreatic carcinoma marker protein GA733-1 and encoded by the gene TACSTD2/GA733-1/M1S1/TROP2, is a protein that may function as a growth factor receptor and is expressed primarily in the placenta, lung and epithelial tissues but also found in certain cancers. The protein was first identified as a carcinoma antigen by the monoclonal GA733. It is a plasma membrane bound protein that transduces an intracellular calcium signal after binding unknown ligands. Mutations in Trop2 are associated with a rare eye disease leading to blindness. Trop-2 has also recently been shown to be important in regulating fibroblast motility and lung cell proliferation via the regulation of the ERK kinase pathway as well as several critical components of the cell migration apparatus.

免疫原

Recombinant protein corresponding to the extracellular domain of human Trop2.

應用

Immunocytochemistry Analysis: A representative lot detected Trop2 in MTE 4-14/T2 and MCF7 cells (Prof. Saverio Alberti, Oncoxx Biotec.).

Flow Cytometry Analysis: A representative lot detected Trop2 in transfected cells, Skov-3 cancer cells, and HT29 cancer cells (Prof. Saverio Alberti, Oncoxx Biotec.).

Inhibition Analysis: A representative lot inhibited tumor growth in DU145 prostate cancer cells, and in HT-29, HCT116 and Colo205 cells. (Note: When used together, Cat. No. MABC519, Anti-Trop2, clone 2G10, Azide Free and Cat. No. MABC518, Anti-Trop2, clone 2EF, Azide Free maximizes inhibition of tumor growth in vivo.) (Prof. Saverio Alberti, Oncoxx Biotec.).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Trop2 antibody, clone 2G10, Azide Free is validated for use in ICC, flow cytometry & inhibition for the detection of Trop2.

品質

Evaluated by Immunocytochemistry Analysis in MET cells transfected with a vector expressing Trop2 or with an empty vector.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Trop 2 in MET cells transfected with a vector expressing Trop2 and not in MET cells transfected with an empty vector.

標靶描述

36 kDa calculated

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable