推荐产品
生物源
mouse
品質等級
抗體表格
culture supernatant
抗體產品種類
primary antibodies
無性繁殖
11B2-1A4, monoclonal
物種活性
rat
製造商／商標名
Chemicon®
技術
immunohistochemistry: suitable (paraffin)
western blot: suitable
同型
IgG1
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
rat ... Cyp11B1(500892)
特異性
Rat p450 11beta-Hydroxylase
免疫原
Aa 272-285 (KNVYRELAE GRQQS-C)
應用
Anti-Cytochrome P450 11β-Hydroxylase Antibody, clone 11B2-1A4 is an antibody against Cytochrome P450 11β-Hydroxylase for use in IH(P) & WB.
Research Category
Metabolism
Metabolism
Research Sub Category
Enzymes & Biochemistry
Enzymes & Biochemistry
Western blot: 1:300
Immunohistochemistry (S.T.F. on paraffin embedded tissue required, traditional formalin fixation or paraformaldehyde fixation is not recommended. Microwave or pressure cooker antigen retrieval; blocking with a solution of 0.5% SDS, 2% milk and 5% NGS in Tris-HCL pH 7.6. Primary antibody is diluted in PBS or TBS typically with no SDS or NGS: 1:50-200, usually overnight refrigerated. After washing 4X with Tris buffer pH 7.6, secondary goat anti-mouse secondary (AP124B) is incubated (1:500-1:2000) diluted in a solution of 2% milk, 5% NGS, Tris-HCL pH 7.6 and 0.05% tween 20. Detection is via streptavidin HRP/DAB. Recommended counter stain is Gill′s Haematoxylin.
Optimal working dilutions must be determined by end user.
STF fixative is available from streck laboratories, www.streck.com).
Immunohistochemistry (S.T.F. on paraffin embedded tissue required, traditional formalin fixation or paraformaldehyde fixation is not recommended. Microwave or pressure cooker antigen retrieval; blocking with a solution of 0.5% SDS, 2% milk and 5% NGS in Tris-HCL pH 7.6. Primary antibody is diluted in PBS or TBS typically with no SDS or NGS: 1:50-200, usually overnight refrigerated. After washing 4X with Tris buffer pH 7.6, secondary goat anti-mouse secondary (AP124B) is incubated (1:500-1:2000) diluted in a solution of 2% milk, 5% NGS, Tris-HCL pH 7.6 and 0.05% tween 20. Detection is via streptavidin HRP/DAB. Recommended counter stain is Gill′s Haematoxylin.
Optimal working dilutions must be determined by end user.
STF fixative is available from streck laboratories, www.streck.com).
外觀
Supernatant. Liquid in 0.1% sodium azide.
儲存和穩定性
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Resveratrol stimulates cortisol biosynthesis by activating SIRT-dependent deacetylation of P450scc.
Donghui Li,Eric B Dammer,Marion B Sewer
Endocrinology null
Chuanming Xu et al.
American journal of physiology. Cell physiology, 311(4), C686-C695 (2016-08-19)
(Pro)renin receptor (PRR) is predominantly expressed in the collecting duct (CD) with unclear functional implication. It is not known whether CD PRR is regulated by high potassium (HK). Here, we aimed to investigate the effect of HK on PRR expression
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