推荐产品
生物源
mouse
品質等級
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
9C3, monoclonal
物種活性
rat, human, mouse
製造商／商標名
Chemicon®
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG2b
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... NCSTN(23385)
特異性
Nicastrin.
免疫原
Synthetic peptide corresponding to the C terminal 18 a.a. of mouse Nicastrin. Due to sequence similarity, it is expected that the antibody will react with most mammals.
應用
Anti-Nicastrin Antibody is an antibody against Nicastrin for use in IP, WB & IC.
Western blot. The antibody recognizes a doublet of ~100-110 kDa. Suggested antibody dilution buffer is TBS with 0.1% Tween-20 and 5% non-fat milk powder. Suggested incubation time is overnight at 2-8°C or 1-2 hours at room temperature.
Immunocytochemistry
Immunoprecipitation. Suggested tissue/cell lysis buffer is 1% Triton X100 or 2% CHAPS. Suggested final reaction volume is 1000 μL with a final protein concentration in the reaction mix of 1 mg/mL. Suggested incubation time is overnight at 2-8°C with rotating. The antibody is known to co-precipitate in CHAPS: presenilin, Aph-1 and Pen-2. Optimal working dilutions must be determined by end user.
Immunocytochemistry
Immunoprecipitation. Suggested tissue/cell lysis buffer is 1% Triton X100 or 2% CHAPS. Suggested final reaction volume is 1000 μL with a final protein concentration in the reaction mix of 1 mg/mL. Suggested incubation time is overnight at 2-8°C with rotating. The antibody is known to co-precipitate in CHAPS: presenilin, Aph-1 and Pen-2. Optimal working dilutions must be determined by end user.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Identification of two novel synaptic ?-secretase associated proteins that affect amyloid ?-peptide levels without altering Notch processing.
Susanne Frykman,Yasuhiro Teranishi,Ji-Yeun Hur,Anna Sandebring,Natsuko Goto Yamamoto et al.
Neurochemistry International null
Cary Esselens et al.
The Journal of cell biology, 166(7), 1041-1054 (2004-09-29)
Presenilin 1 (PS1) interacts with telencephalin (TLN) and the amyloid precursor protein via their transmembrane domain (Annaert, W.G., C. Esselens, V. Baert, C. Boeve, G. Snellings, P. Cupers, K. Craessaerts, and B. De Strooper. 2001. Neuron. 32:579-589). Here, we demonstrate
Jolanta L Lundgren et al.
Journal of neurochemistry, 135(3), 606-615 (2015-08-25)
Synaptic degeneration and accumulation of the neurotoxic amyloid β-peptide (Aβ) in the brain are hallmarks of Alzheimer disease. Aβ is produced by sequential cleavage of the amyloid precursor protein (APP), by the β-secretase β-site APP cleaving enzyme 1 (BACE1) and
gamma-Secretase dependent production of intracellular domains is reduced in adult compared to embryonic rat brain membranes.
Fr?nberg, J; Karlstrom, H; Winblad, B; Tjernberg, LO; Frykman, S
Testing null
Synaptic and endosomal localization of active gamma-secretase in rat brain.
Frykman, S; Hur, JY; Fr?nberg, J; Aoki, M; Winblad, B; Nahalkova, J; Behbahani, H; Tjernberg, LO
Testing null
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