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Merck
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Key Documents

MAB4301C3

Sigma-Aldrich

Anti-SSEA1 Antibody, clone MC-480, Cy3 conjugate

clone MC-480, from mouse, CY3 conjugate

别名:

SSEA-1, SSEA1, CD15, Lewis X, 3-FAL

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

共軛

CY3 conjugate

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

MC-480, monoclonal

物種活性

human

技術

immunocytochemistry: suitable

同型

IgM

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... FUT4(2526)

一般說明

Stage-specific embryonic antigen-1 (SSEA-1) is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

特異性

This antibody recognizes mouse Stage-Specific Embryonic Antigen-1 (SSEA-1).

免疫原

F9 teratocarcinoma stem cells (X-irradiated).

應用

Detect SSEA1 using this Anti-SSEA1 Antibody, clone MC-480, Cy3 conjugate validated for use in ICC.
Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected no SSEA-1 in H9 human embryonic stem cells.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Glycobiology

品質

Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected SSEA-1 in mouse embryonic stem cells (SCR012).

標靶描述

The unconjugated parent antibody (Catalog No. MAB4301) has an observed MW at 220-240 kDa

外觀

Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

儲存和穩定性

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

分析報告

Control
Mouse embryonic stem cells (SCR012).

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Bo Feng et al.
Nature cell biology, 11(2), 197-203 (2009-01-13)
The dominant effect of transcription factors in imparting expanded potency is best exemplified by the reprogramming of fibroblasts to pluripotent cells using retrovirus-mediated transduction of defined transcription factors. In the murine system, Oct4, Sox2, c-Myc and Klf4 are sufficient to
Establishment and characterization of embryonic stem-like cells from porcine somatic cell nuclear transfer blastocysts.
Kim S, Kim JH, Lee E, Jeong YW, Hossein MS, Park SM, Park SW, Lee JY, Jeong YI, Kim HS et al.
Zygote null
Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes.
Sung LY, Chang CC, Amano T, Lin CJ, Amano M, Treaster SB, Xu J, Chang WF, Nagy ZP, Yang X, Tian XC
Cellular Reprogramming null

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