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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
8A11.2, monoclonal
物種活性
dog (Wang, et al 2001), rabbit (Chaytor, et al 2005), chicken (Elenes, et al 2001), mouse, human, rat (Sorensen, et al 2008)
製造商／商標名
Chemicon®
技術
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG1
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
dog ... Gjc1(490936)
human ... GJC1(10052)
mouse ... Gjc1(14615)
rat ... Gjc1(266706)
一般說明
Connexin 45 is member of a family of gap junction proteins known as Connexin gene family. It is expressed in a wide variety of cell types during development and adult life, particularly in cardiac and smooth muscle tissues as well as brain tissues. Connexin 45 forms part of a network of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. There a number of alternatively spliced transcript variants that all seen to encode the same protein isoform.
特異性
Clone 8A11.2 is highly specific for Connexin 45 protein with no cross reactivity as analyzed by western blotting to any other connexin family member.
Others species not tested. Also confirmed to work on rat C6 cells.
免疫原
Epitope: near C-terminus, cytoplasmic
The peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-367 of human connexin 45.
應用
Anti-Connexin 45 Antibody, near C-terminus, cytoplasmic, clone 8A11.2 is an antibody against Connexin 45 for use in IC, IH & WB.
Immunblotting (~46 kDa): 0.5 -1 µg/mL with ECL detection. Samples should be prepared with proteinase inhibitors and kept cold for best results. Alkali extraction methods can be used to enrich for connexin protein fractions. Briefly, 1 ml of NaHCO3 containing protease and phosphatase inhibitors and 22 µl of 1 M NaOH were added to frozen cell pellets. This cell suspension was sonicated for 30 s and incubated on ice for 50 min. Then, the homogenate was centrifuged for 30 min at 30,000 X g at 4°C. The supernatant was discarded, and the pellet was resuspended in sample buffer. {Elenes, S et al 2001}. 8% SDS_PAGE gels and PVDF membranes are suggested for greatest clarity.
Immunohistochemistry: fresh frozen, methanol fixed sections {Ujiie H et al (2003)}; 4% fixed frozen sections {Ikeda, Y et al 2007). 1-10 µg/mL with overnight incubations; 4% PFA fixed, paraffin-embedded sections {Gluhak-Heinrich, J et al 2007}, 1:50 with enzymatic detection.
Immunocytochemistry: 2% PFA fixed cells (15’, RT), permeabilized with a solution of 0.2% triton X-100/4% BSA {Sorensen et al 2008}.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: fresh frozen, methanol fixed sections {Ujiie H et al (2003)}; 4% fixed frozen sections {Ikeda, Y et al 2007). 1-10 µg/mL with overnight incubations; 4% PFA fixed, paraffin-embedded sections {Gluhak-Heinrich, J et al 2007}, 1:50 with enzymatic detection.
Immunocytochemistry: 2% PFA fixed cells (15’, RT), permeabilized with a solution of 0.2% triton X-100/4% BSA {Sorensen et al 2008}.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
外觀
Format: Purified
Liquid at 1 mg/mL in 0.02M PB with 0.25M NaCl,pH=7.6 containing 0.1% sodium azide as a preservative.
儲存和穩定性
Maintain at 2° to 8°C in undiluted aliquots for up to 12 months fromd date of receipt. Prevent multiple warming and coolings. Aliquot in tightly closed tubes to prevent loss.
分析報告
Control
Cardiac tissue, particularly sinoatrial tissues.
Cardiac tissue, particularly sinoatrial tissues.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Chul-Kyun Chung et al.
Cell and tissue research, 329(2), 295-300 (2007-04-24)
Connexins are gap-junction proteins forming hexameric structures in the plasma membranes of adjacent cells, thereby creating intercellular channels. Connexin 43 (CX43) is expressed in pulp tissue. However, its function in dental pulp tissue has yet to be fully investigated. We
Essential role of Gap junctions in NO- and prostanoid-independent relaxations evoked by acetylcholine in rabbit intracerebral arteries.
Ujiie, Hiroshi, et al.
Stroke, 34, 544-550 (2003)
Heterotypic docking of Cx43 and Cx45 connexons blocks fast voltage gating of Cx43.
Elenes, S, et al.
Biophysical Journal, 81, 1406-1418 (2001)
Connexin-mimetic peptides dissociate electrotonic EDHF-type signalling via myoendothelial and smooth muscle gap junctions in the rabbit iliac artery.
Chaytor, Andrew T, et al.
British Journal of Pharmacology, 144, 108-114 (2005)
Role of gap junctions in spontaneous activity of the rat bladder.
Ikeda, Y, et al.
American Journal of Physiology: Renal Physiology, 293, F1018-F1025 (2007)
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