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Merck
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Key Documents

GR15

Sigma-Aldrich

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

liquid, clone EGFR.1, Calbiochem®

别名:

Anti-Epidermal Growth Factor Receptor

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

100

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

EGFR.1, monoclonal

形狀

liquid

包含

≤0.1% sodium azide as preservative

物種活性

human

應無反應活性

rat, mouse

製造商／商標名

Calbiochem®

儲存條件

do not freeze

同型

IgG2b

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

基因資訊

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

一般說明

Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, and IP.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
Recognizes the ~170 kDa EGF receptor protein in A431 cells. For neutralization studies Cat. No. GR15L is recommended.

免疫原

Human
partially purified, human EGFR from A431 cells

應用


Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; use Cat. No. GR15L; see application references)

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

外觀

In 50 mM sodium phosphate, 0.2% gelatin, pH 7.5.

分析報告

Negative Control
HL-60 cells
Positive Control
A-431 cells

其他說明

EGF-Receptor (Ab-5) binds to A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
Hunter T., 1984. Nature311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

nwg

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Jean-Philippe Grossier et al.
The EMBO journal, 33(1), 35-45 (2013-12-25)
Preferred sites of endocytosis have been observed in various cell types, but whether they occur randomly or are linked to cellular cues is debated. Here, we quantified the sites of endocytosis of transferrin (Tfn) and epidermal growth factor (EGF) in

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