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Merck
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Key Documents

GR01L

Sigma-Aldrich

Anti-EGFR (Ab-1) Mouse mAb (528)

lyophilized, clone 528, Calbiochem®

别名:

Anti-Epidermal Growth Factor Receptor

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

528, monoclonal

形狀

lyophilized

不包含

preservative

物種活性

human

應無反應活性

rat, mouse

製造商/商標名

Calbiochem®

儲存條件

OK to freeze

同型

IgG2a

運輸包裝

ambient

儲存溫度

2-8°C

目標翻譯後修改

unmodified

基因資訊

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

一般說明

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.
Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa EGFR protein.
Recognizes the EGF receptor protein in A431 cells and normal skin.

免疫原

partially purified, human EGFR from human A431 cells

應用

Flow Cytometry (5 µg/ml)

Frozen Sections (2.5 µg/ml)

Immunofluorescence (5 µg/ml)

Immunoprecipitation (1 µg/sample)

Neutralization Studies (see comments and application references)

Paraffin Sections (see application references)

警告

Toxicity: Standard Handling (A)

外觀

Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.

重構

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.

分析報告

Positive Control
A-431 cells or skin tissue

其他說明

Hunter T. 1984. Nature311, 414.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1


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