推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
DCS-6, monoclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human, mouse, rat
製造商／商標名
Calbiochem®
儲存條件
do not freeze
同型
IgG2a
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... CCND1(595)
mouse ... Ccnd1(12443)
rat ... Ccnd1(58919)
一般說明
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein.
Recognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.
This Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of Cyclin D1 (Ab-3).
免疫原
Human
recombinant human cyclin D1
應用
Flow Cytometry (5 µg/ml; Cat. No. CC12, CC12F)
Frozen Sections (see application references)
Immunoblotting (1 µg/ml)
Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)
Immunoprecipitation (see application references)
Neutralization Studies (see application references)
Paraffin Sections (5 µg/ml, heat pre-treatment required)
Frozen Sections (see application references)
Immunoblotting (1 µg/ml)
Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)
Immunoprecipitation (see application references)
Neutralization Studies (see application references)
Paraffin Sections (5 µg/ml, heat pre-treatment required)
包裝
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外觀
In 50 mM sodium phosphate buffer, 0.2% gelatin.
分析報告
Negative Control
trp E (Ab-1)
trp E (Ab-1)
Positive Control
MCF7, or A431 cells or breast carcinoma tissue
MCF7, or A431 cells or breast carcinoma tissue
其他說明
Does not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.
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Jiang, W., et al. 1992. Cancer Res.2, 2980.
Pines, J. 1992. Cell Growth Differ.2, 305.
Xiong, Y., et al. 1992. Genomics13, 575.
Xiong, Y., et al. 1992. Cell71, 504.
Motokura, T., et al. 1991. Nature350, 512.
Solomon, M.J., et al. 1990. Cell63, 1013.
Draetta, G., and Beach, D. 1988. Cell54, 17.
Jiang, W., et al. 1992. Cancer Res.2, 2980.
Pines, J. 1992. Cell Growth Differ.2, 305.
Xiong, Y., et al. 1992. Genomics13, 575.
Xiong, Y., et al. 1992. Cell71, 504.
Motokura, T., et al. 1991. Nature350, 512.
Solomon, M.J., et al. 1990. Cell63, 1013.
Draetta, G., and Beach, D. 1988. Cell54, 17.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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