CBL202

Anti-Vimentin Antibody, clone VIM 3B4

clone VIM 3B4, Chemicon^®, from mouse

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: VIM 3B4, monoclonal
• 物種活性: chicken, amphibian, bovine, monkey, canine, human
• 製造商／商標名: Chemicon^®
• 技術: ELISA: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG2a
• NCBI登錄號: NM_003380.2
• UniProt登錄號: P08670
• 運輸包裝: wet ice
• 基因資訊: human ... VIM(7431)

说明

特異性

Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This antibody is positive with cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. It can also be used to detect coexpression of vimentin in combination with other intermediate filament proteins. VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Polypeptide reacting: MW 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.

免疫原

Vimentin purified from bovine lens

應用

Western blot
Immunohistochemistry: 1:100; Frozen and paraffin-embedded tissue sections with a minimum of a one hour incubation (longer for paraffin); protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence
ELISA

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin.

標靶描述

57 kDa

外觀

Protein A purified

Format: Purified

Protein A Purified immunoglobulin presented as lyophilized material. Reconstitute with 1ml distilled water. Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.

儲存和穩定性

Maintain at 2–8°C for up to 12 months from date of receipt.

分析報告

Control
Positive in RD cells, glioma cells, fibroblasts (SV-80), and MDCK cell line

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H312 + H332,H412
• 防範說明: P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312
• 危險分類: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3