AC113

Anti-Neomycin Phosphotransferase II Antibody | AC113

from rabbit, purified by affinity chromatography

• 别名: Aminoglycoside 3′-phosphotransferase, APH(3′)-II, APH(3′)II, Kanamycin kinase, type II, Neomycin-kanamycin phosphotransferase type II
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 技術: western blot: suitable
• NCBI登錄號: YP_788126
• UniProt登錄號: P00552
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified

说明

一般說明

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin^®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

特異性

This antibody reacts to lysates from cells transfected or transformed with a plasmid containing a kanamycin/neomycin resistance gene, nptII (Aminoglycoside 3′-phosphotransferase).

免疫原

Recombinant protein corresponding to full length neomycin phosphotransferase (NPT) II.

應用

Research Category
Secondary & Control Antibodies

Research Sub Category
Kinases & Phosphatases

品質

Evaluated by Western Blot in untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Neomycin phosphotransferase II in 10 µg of neomycin treated HEK293 cells transfected with pUSEamp(-).

標靶描述

~29 kDa observed.

外觀

Affinity purified

Hek 293 +/- transfection with pUSEamp(-)and neomycin treatment.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).

法律資訊

Geneticin is a registered trademark of Gibco BRL Life Technologies, Inc.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable