ABT1340
Anti-Myosin-10/MYH10/Myosin IIB Antibody
from rabbit, purified by affinity chromatography
别名:
Myosin-10, Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, NMMHC-B, NMMHC II-b, NMMHC-IIB, Non-muscle myosin heavy chain B, Non-muscle myosin heavy chain Iib, MHC IIB
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所有图片(2)
About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
推荐产品
生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
human, mouse
物種活性（以同源性預測）
rat (based on 100% sequence homology)
技術
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... MYH10(4628)
一般說明
Myosin-10 (UniProt P35580; also known as Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, NMMHC-B, NMMHC II-b, NMMHC-IIB, Non-muscle myosin heavy chain B, Non-muscle myosin heavy chain IIb) is encoded by the MYH10 (also known as NMMHCB) gene (Gene ID 4628) in human. Nonmuscle myosin II plays an important role in cell migration and invasion. The myosin II holoenzyme consists of two myosin heavy chains (MHCs), two essential light chains, and two regulatory light chains. Three different nonmuscle MHC II proteins exist in mammals, IIA (MYH9), IIB (MYH10), and IIC (MYH14), with both unique and overlapping roles. One of the initial steps in cancer cell metastasis is thought to be the process of epithelial–mesenchymal transition (EMT). Myosin IIB expression and myosin IIA heavy chain phosphorylation are upregulated upon TGF-β–induced EMT in mammary epithelial cells, while inhibition of myosin IIB expression in post-EMT mesenchymal cells reduces transmigration and invasion.
特異性
Expected to react with all five spliced isoforms of human Myosin-10 reported by UniProt (P35580).
免疫原
KLH-conjugated linear peptide corresponding to the C-terminal sequence of human myosin-10.
應用
Anti-Myosin-10/MYH10/Myosin IIB Antibody is an antibody against Myosin-10/MYH10/Myosin IIB for use in Western Blotting, Immunocytochemistry, Immunofluorescence.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Myosin-10/MYH10/Myosin IIB in 10 µg of MCF-10A cell lysate.
Western Blotting Analysis: A representative lot detected elevated MHC IIB expression in MDA-MB-468, MDA-MB-231, and MCF10A, while very low MHC IIB expression was detected in MCF7, T47D, BT-474, and SKBR3 (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Western Blotting Analysis: A representative lot detected MHC IIB levels in NMuMG murine mammary gland cells upon TGF-beta stimulation or shRNA-mediated hnRNP E1 knockdown. E1 overexpression obolished TGF-beta stimulated MHC IIB upregulation in NMuMG cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Immunocytochemistry Analysis: A representative lot detected MHC IIB immunoreactivity localized to the rear and perinuclear regions of migrating and transmigrating hnRNP E1-knockdown murine mammary gland E1-shRNA NMuMG cells by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% TritonX-100-permeablized cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Immunofluorescence Analysis: A representative lot detected MHC IIB immunoreactivity predominantly in the SAM-expressing myoepithelial layer, but not the cytokeratin 8-expressing luminal layer, in 4% paraformaldehyde-fixed, paraffin-embedded mouse mammary tissue sections by fluorescent immunohistochemstry (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Western Blotting Analysis: A representative lot detected elevated MHC IIB expression in MDA-MB-468, MDA-MB-231, and MCF10A, while very low MHC IIB expression was detected in MCF7, T47D, BT-474, and SKBR3 (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Western Blotting Analysis: A representative lot detected MHC IIB levels in NMuMG murine mammary gland cells upon TGF-beta stimulation or shRNA-mediated hnRNP E1 knockdown. E1 overexpression obolished TGF-beta stimulated MHC IIB upregulation in NMuMG cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Immunocytochemistry Analysis: A representative lot detected MHC IIB immunoreactivity localized to the rear and perinuclear regions of migrating and transmigrating hnRNP E1-knockdown murine mammary gland E1-shRNA NMuMG cells by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% TritonX-100-permeablized cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Immunofluorescence Analysis: A representative lot detected MHC IIB immunoreactivity predominantly in the SAM-expressing myoepithelial layer, but not the cytokeratin 8-expressing luminal layer, in 4% paraformaldehyde-fixed, paraffin-embedded mouse mammary tissue sections by fluorescent immunohistochemstry (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
品質
Evaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myosin-10/MYH10/Myosin IIB in 10 µg of A549 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myosin-10/MYH10/Myosin IIB in 10 µg of A549 cell lysate.
標靶描述
~250 kDa observed. 229.0 kDa (isoform 1), 230.8 kDa (isoform 2), 231.4 kDa (isoform 3), 232.5 kDa (isoform 4), 230.0 kDa (isoform 5) calculated. Uncharacterized band(s) may appear in some lysates.
其他說明
Concentration: Please refer to lot specific datasheet.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Yu Chen et al.
Nature methods, 18(5), 528-541 (2021-05-05)
Human pluripotent stem cells (hPSCs) are capable of extensive self-renewal yet remain highly sensitive to environmental perturbations in vitro, posing challenges to their therapeutic use. There is an urgent need to advance strategies that ensure safe and robust long-term growth
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