ABE101-S

Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size

from rabbit, purified by affinity chromatography

• 别名: H3K9me1, Histone H3 (monomethyl K9), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 技術: dot blot: suitable; immunocytochemistry: suitable; inhibition assay: suitable (peptide); western blot: suitable
• NCBI登錄號: NP_003484.1
• UniProt登錄號: Q16695
• 運輸包裝: wet ice
• 目標翻譯後修改: monomethylation (Lys9)
• 基因資訊: human ... HIST1H3F(8968)

说明

一般說明

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

特異性

Broad species cross-reactivity is expected based on sequence homology.

This antibody recognizes Histone H3 methylated at Lys9.

免疫原

Epitope: Lys9

KLH-conjugated branched peptide corresponding to human Histone H3 methylated at Lys9.

應用

Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated on lysine 9. Also known as H3K9me1, this antibody has dot blot proven specificity, and has been validated in WB, ICC, PIA. Small pack size is ideal for screening.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in HeLa and A431 cells.

Peptide Inhibition Analysis: 1:10,000 dilution from a representative lot was used with HeLa acid extract for peptide block analysis.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

品質

Evaluated by Western Blot in HeLa acid extract lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected Histone H3 on 10 µg of HeLa acid extract lysate.

標靶描述

~ 17 kDa

聯結

Replaces: 07-395

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
HeLa acid extract lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable