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Merck
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Key Documents

ABC344

Sigma-Aldrich

Anti-ATG13 Antibody

from rabbit, purified by affinity chromatography

别名:

Autophagy-related protein 13

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

mouse, rat

物種活性(以同源性預測)

human (immunogen homology)

技術

immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... ATG13(9776)
mouse ... Atg13(51897)

一般說明

Autophagy-related protein 13 (ATG13) is an autophagy factor that is involved in the preservation of cellular nutrients under starvation conditions as well as under normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. Knockdown of ATG13 inhibits autophagosome formation.

免疫原

KLH-conjugated linear peptide corresponding to human ATG13.

應用

Anti-ATG13 Antibody is a highly specific rabbit polyclonal antibody, that targets ATG13 & has been tested in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected ATG13 in mouse heart tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected ATG13 in mouse heart cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

品質

Evaluated by Western Blotting/Peptide Inhibition Assay in rat heart tissue lysate.

Western Blotting/Peptide Inhibition Assay Analysis: 1 µg/mL of this antibody detected ATG13 in 15 µg of rat heart tissue lysate.

標靶描述

~49 kDa observed. Uniprot describes 5 isoforms ranging between ~44 kDa and ~60 kDa Uncharacterized bands may appear in some lysate(s).

外觀

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.1% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Rat heart tissue lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Christopher J Shoemaker et al.
PLoS biology, 17(4), e2007044-e2007044 (2019-04-02)
The power of forward genetics in yeast is the foundation on which the field of autophagy research firmly stands. Complementary work on autophagy in higher eukaryotes has revealed both the deep conservation of this process, as well as novel mechanisms

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