推荐产品
生物源
rabbit
品質等級
抗體表格
fractionated antiserum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
human
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunofluorescence: suitable
適合性
not suitable for Western blot
not suitable for immunohistochemistry (Paraffin)
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... COL3A1(1281)
特異性
The antibody reacts with native and heat denatured (non-reduced) human collagen type III. Cross reactions with other types of collagen under native conditions do occur. There is 10% cross reactivity with human collagen type I, 2% cross reactivity with human collagen type II, and 4% cross reactivity with human collagen type IV and V. There is no cross reactivity with other human plasma proteins under native ELISA conditions. Reactivity under denatured conditions has not been examined.
Cross Reactivity Percent
Human collagen type I 10%
Human collagen type II 2%
Human collagen type III 100%
Human collagen type IV and V 4%
Human plasma proteins do not interfere with binding to collagen.
Cross Reactivity Percent
Human collagen type I 10%
Human collagen type II 2%
Human collagen type III 100%
Human collagen type IV and V 4%
Human plasma proteins do not interfere with binding to collagen.
免疫原
Human placental collagen type III
應用
ELISA 1:3,000
Indirect immunofluorescence on cryostat sections or cultured cells 1:20-1:40
Optimal working dilutions must be determined by the end user.
Indirect immunofluorescence on cryostat sections or cultured cells 1:20-1:40
Optimal working dilutions must be determined by the end user.
This Anti-Collagen Type III Antibody is validated for use in ELISA, IF for the detection of Collagen Type III.
外觀
500ul containing of 0.15M NaCl, 10 mM sodium phosphate pH 7.5, with 0.1% mannitol and o.1% dextran as stabilizers contains no preservatives.
Immunoglobulin fraction was prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiserum cross-absorbed over immobilized human serum proteins, immunoglobulins, and collagen types I, II, IV and V.
Immunoglobulin fraction was prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiserum cross-absorbed over immobilized human serum proteins, immunoglobulins, and collagen types I, II, IV and V.
Format: Purified
儲存和穩定性
Antibody is stable for 12 months from date of receipt when stored at 2-8ºC. Remove any nondispersed aggregates by microcentrifugation prior to use.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Shlomit Mendelboum Raviv et al.
Thrombosis research, 129(4), e29-e35 (2011-11-08)
Von Willebrand factor (VWF) and platelet binding needs a uniform collagen matrix therefore we aimed to find an optimal condition for the preparation of human type-I and type-III collagen matrices. The effects of pH, salt and ligand concentration and binding
The fetal fibroblast: the effector cell of scarless fetal skin repair.
H P Lorenz, R Y Lin, M T Longaker, D J Whitby, N S Adzick
Plastic and Reconstructive Surgery null
Chronic hibernating myocardium: interstitial changes.
J Ausma, J Cleutjens, F Thone, W Flameng, F Ramaekers, M Borgers
Molecular and Cellular Biochemistry null
Fibrillin microfibrils are reduced in skin exhibiting striae distensae.
R E Watson, E J Parry, J D Humphries, C J Jones, D W Polson, C M Kielty, C E Griffiths et al.
British Journal of Dermatology null
Emma J Watts et al.
Research in veterinary science, 92(2), 213-218 (2011-04-13)
Wounds on the limbs of horses are notoriously difficult to heal, with over production of TGFβ1 thought to be responsible for excessive scarring; in contrast, wounds in the oral cavity heal rapidly with minimal scarring. This experiment aimed to determine
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