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Merck
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主要文件

AB3832

Sigma-Aldrich

Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific

Chemicon®, from rabbit

别名:

Anti-EBP50, Anti-NHERF, Anti-NHERF-1, Anti-NHERF1

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

monkey, human, mouse, rat

製造商/商標名

Chemicon®

技術

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

phosphorylation (pThr567)

基因資訊

human ... SLC9A3R1(9368)

特異性

Recognizes Thr567 phosphorylated ezrin, Thr564 phosphorylated radixin, and Thr558 phosphorylated moesin. Antibody does not cross-react with other related phospho proteins such as merlin or band 4.1.

免疫原

Epitope: phospho-specific
KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Thr567 of human ezrin

應用

Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific detects level of Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) & has been published & validated for use in IH(P) & WB.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Western blotting 1:1,000 (for best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight). The antibody recognizes 75KDa (Moesin, and ~80kDa Ezrln & Radixin) bands.

Immunohistochemistry (paraffin): 1:50

Optimal working dilutions must be determined by the end user.

標靶描述

75KDa Moesin, and ~80kDa Ezrin & Radixin

外觀

ImmunoAffinity Purified
Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/ml BSA, with 50% glycerol.

儲存和穩定性

Maintain at -20°C for 12 months, do not aliquot.

分析報告

Control
Cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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Minji Kim et al.
Journal of cell science, 128(23), 4317-4327 (2015-10-21)
Tubulogenesis is fundamental to the development of many epithelial organs. Although lumen formation in cysts has received considerable attention, less is known about lumenogenesis in tubes. Here, we utilized tubulogenesis induced by hepatocyte growth factor (HGF) in MDCK cells, which

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