AB3212-I

Anti-Na+/HCO3- Cotransporter Antibody

from rabbit, purified by affinity chromatography

• 别名: Electrogenic sodium bicarbonate cotransporter 1, Sodium bicarbonate cotransporter, Na(+)/HCO3(-) cotransporter, Solute carrier family 4 member 4, kNBC1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: rat, human
• 物種活性（以同源性預測）: rabbit (based on 100% sequence homology)
• 技術: immunohistochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_001091954
• UniProt登錄號: Q9Y6R1
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SLC4A4(8671)

说明

一般說明

Electrogenic sodium bicarbonate cotransporter 1 (UniProt Q9Y6R1; also known as Sodium bicarbonate cotransporter, Na(+)/HCO3(-) co-transporter, Solute carrier family 4 member 4, kNBC1) is encoded by the SLC4A4 (also known as NBC, NBC1, NBCE1) gene (Gene ID 8671) in human. Bicarbonate (HCO3-) transporters are the principal regulators of pH in cells and play a vital role in acid-base movement in various tissues. There exist four types of HCO3- transporters, namely Cl-/-HCO3- exchangers, Na+/HCO3- co-transporters (NBCs), a K+/HCO3- co-transporter, and a Na+-driven Cl-/-HCO3- exchanger. SLC4A4 is a multi-pass membrane NBC with 10 transmembrane segments, 5 extracellular domains, and 6 intracellular domains, having both its N- and C-terminal (a.a. 1-468 and a.a. 987-1079, respectively) exposed intracellularly. NBCs mediate the coupled movement of sodium and bicarbonate ions, in a 3 to 1 stoichiometric ratio, across the plasma membrane. Multiple isoforms are reported as a result of alternative splicing, including NBCe1-A (kNBC1, kNBC-1; UniProt Q9JI66-2/Rat and Q9Y6R1-2/Human) that is mainly expressed in the kidney, NBCe1-B (bNBC1, pNBC-1; UniProt Q9JI66-1/Rat and Q9Y6R1-1/Human) expressed in the pancreas and other tissues, and NBCe1-C (b2NBC; UniProt Q9JI66-3/Rat and Q9Y6R1-5/Human) expressed in the brain.

特異性

Expected to react with all rat and human spliced isoforms reported by UniProt (Q9JI66 and Q9Y6R1, respectively).

免疫原

Epitope: Amino acids 338-391

GST-tagged recombinant protein corresponding to amino acids 338-391 of human Na+/HCO3- cotransporter.

應用

Detect Na+/HCO3- Cotransporter using this Anti-Na+/HCO3- Cotransporter Antibody validated for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Na+/HCO3- cotransporter in human cerebral cortex, kidney and pancreas tissues.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

品質

Evaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Na+/HCO3- cotransporter in 10 µg of human kidney tissue lysate.

標靶描述

~130 kDa observed. Uncharacterized band(s) may appear in some lysates.

外觀

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable