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Key Documents

70836-M

Sigma-Aldrich

Origami B Competent Cells - Novagen

Escherichia coli, rod shaped

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About This Item

分類程式碼代碼:
41106202
NACRES:
NA.81

product name

Origami B Competent Cells - Novagen, Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.

生物源

Escherichia coli

品質等級

製造商/商標名

Novagen®

儲存條件

OK to freeze

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

細胞轉化

transformation efficiency: >2×106 cfu/μg

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

Genotype: F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC gor522::Tn10 trxB (KanR, TetR)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Origami B host strains carry the same trxB/gor mutations as the original Origami strains, except that they are derived from a lacZY mutant of BL21. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner and Origami hosts in one strain background. The trxB and gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, these strains are recommended for use only with pET plasmids carrying the ampicillin resistance marker bla.
Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.

成分

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlOrigami B Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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