69725-M

λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen

• 分類程式碼代碼: 41106200
• NACRES: NA.51

属性

• 製造商／商標名: Novagen^®
• 品質等級: 100
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 儲存溫度: −70°C

说明

一般說明

The λDE3 Lysogenization Kit is designed for site-specific integration of λDE3 prophage into an E. coli host cell chromosome, such that the lysogenized host can be used to express target genes cloned in pET vectors. λDE3 is a recombinant phage carrying the cloned gene for T7 RNA polymerase under lacUV5 control. Lysogens are prepared by co-infection with the three provided phages, and can be verified by plating the T7 Tester Phage (a T7 RNA polymerase deletion mutant). This phage is unable to make a plaque on cells that lack T7 RNA polymerase, but makes normal plaques on λDE3 lysogens in the presence of IPTG.The T7 RNA Polymerase Monoclonal Antibody can be used to verify the presence of T7 RNA polymerase in the host strain by Western blot analysis.

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

成分

•200 µlλDE3 Phage Lysate

•200 µlHelper Phage Lysate

•200 µlSelection Phage Lysate

•50 µlT7 Tester Phage Lysate

•200 µlHMS174(DE3) glycerol stock

•1 µgpLysS DNA

•1 µgpLysE DNA

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 10 - Combustible liquids