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Merck
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Key Documents

598502

Sigma-Aldrich

Thioredoxin Reductase, Yeast, Recombinant, E. coli

别名:

ryNTR

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.54

重組細胞

expressed in E. coli

品質等級

化驗

≥95% (SDS-PAGE)

形狀

lyophilized solid

比活性

≥5 units/mg protein

製造商/商標名

Calbiochem®

儲存條件

OK to freeze

溶解度

aqueous buffer, pH 7.0: soluble

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

Recombinant, yeast thioredoxin reductase expressed in E. coli. Yeast thioredoxin reductase is a flavoprotein that has no selenocysteine (SeCys). It catalyzes the NADPH-dependent reduction of thioredoxin as well as other endogenous and exogenous proteins. Plays an important role in many aspects of cell function, including protection against oxidative stress/injury, cell growth and transformation, and the recycling of ascorbate from its oxidized form.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enzyme that will cause an absorbance change of 1 at 412 nm using 200 µM NADPH and 5 mM DTNB, per min at 25°C, pH 7.0.

外觀

Lyophilized from 150 mM NaCl, 20 mM phosphate buffer, pH 7.4.

重構

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for 1 month at 4°C or 3 months at -70°C.

其他說明

Mustacich, D. and Powis, G. 2000. Biochem. J.346, 1.
Wang, P.F., et al. 1998. Biochemistry37, 16378.
Holmgren, A., 1979. J. Biol. Chem. 254, 9627.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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