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重組細胞
expressed in E. coli
品質等級
化驗
≥95% (SDS-PAGE)
形狀
lyophilized solid
比活性
≥5 units/mg protein
製造商/商標名
Calbiochem®
儲存條件
OK to freeze
溶解度
aqueous buffer, pH 7.0: soluble
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
Recombinant, yeast thioredoxin reductase expressed in E. coli. Yeast thioredoxin reductase is a flavoprotein that has no selenocysteine (SeCys). It catalyzes the NADPH-dependent reduction of thioredoxin as well as other endogenous and exogenous proteins. Plays an important role in many aspects of cell function, including protection against oxidative stress/injury, cell growth and transformation, and the recycling of ascorbate from its oxidized form.
警告
Toxicity: Standard Handling (A)
單位定義
One unit is defined as the amount of enzyme that will cause an absorbance change of 1 at 412 nm using 200 µM NADPH and 5 mM DTNB, per min at 25°C, pH 7.0.
外觀
Lyophilized from 150 mM NaCl, 20 mM phosphate buffer, pH 7.4.
重構
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for 1 month at 4°C or 3 months at -70°C.
其他說明
Mustacich, D. and Powis, G. 2000. Biochem. J.346, 1.
Wang, P.F., et al. 1998. Biochemistry37, 16378.
Holmgren, A., 1979. J. Biol. Chem. 254, 9627.
Wang, P.F., et al. 1998. Biochemistry37, 16378.
Holmgren, A., 1979. J. Biol. Chem. 254, 9627.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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