48-610MAG

MILLIPLEX MAP STAT Cell Signaling Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay

• 别名: Magnetic Bead STAT Kit, STAT Cell Signaling Kit
• 分類程式碼代碼: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

属性

• 物種活性: human
• 品質等級: 200
• 製造商／商標名: Milliplex^®
• 技術: multiplexing: suitable
• 檢測方法: fluorometric (Luminex xMAP)
• 儲存溫度: 2-8°C

说明

一般說明

The STAT (Signal Transducers and Activators of Transcription) family, consisting of seven mammalian members, regulates many aspects of growth, survival and differentiation in cells. The extracellular binding of cytokines, hormones, growth factors and neurotransmitters to receptors induces phosphorylation of the intracellular Janus kinases (JAKs) that, in turn, phosphorylate STAT proteins at conserved tyrosine residues, activating SH2-mediated dimerization followed by translocation to the nucleus. STAT dimers then bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes.

特異性

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

應用

• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 μL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).

Research Category
Signaling|Apoptosis & Cancer

The analytes available for this multiplex kit are: STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/699), STAT6 (Tyr641).

Used to detect/quantify:STAT1 (Tyr701)|STAT2 (Tyr690)|STAT3 (Tyr705)|STAT5A/B (Tyr694/699)|STAT6 (Tyr641)

包裝

96-well plate

儲存和穩定性

Recommended storage for kit components is 2 - 8°C.

法律資訊

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

安全信息

• 象形圖: GHS05,GHS07,GHS09
• 訊號詞: Danger
• 危險聲明: H302,H315,H318,H410
• 防範說明: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• 危險分類: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable