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Merck
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文件

48-690MAG

Millipore

MILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000

物種活性

human

品質等級

200

製造商／商標名

Milliplex®

技術

multiplexing: suitable

assay range

inter-assay cv: 4-17%
intra-assay cv: 3-8%

檢測方法

fluorometric (Luminex xMAP)

儲存溫度

2-8°C

相关类别

一般說明

The Milliplex® MAP 7-Plex Human T-Cell Receptor (TCR) Magnetic Bead Kit is used to detect changes in phosphorylated CREB (Ser133), Erk/MAP kinase 1/2 (Thr185/Tyr187), and phosphorylated tyrosine residues on CD3 epsilon chain, Lck, ZAP-70, LAT, and Syk in T-cell lysates using the Luminex® system. Although the protein kinase Syk is not part of the T-Cell Receptor pathway, it does play a significant role in B-Cell Receptor signaling and acts as a negative control for the analysis of T-Cell Receptor signaling. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures.

特異性

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

應用

  • An overnight (4°C) incubation is recommended for best results.
  • This assay requires 25 μL diluted cell lysate.
  • This kit must be run using Assay Buffer 2 (provided).
  • 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).

Research Category
Cell Signaling
This cell signaling panel includes the following analytes in this multiplex kit: CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr).
Used to detect/quantify:CD3ε (pan Tyr)|CREB (S133)|ERK (Thr185/Tyr187)|LAT (pan Tyr)|Lck (pan Tyr)|Syk (pan Tyr)|ZAP-70 (pan Tyr)

儲存和穩定性

Recommended storage for kit components is 2 - 8°C

法律資訊

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

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