推荐产品
重組細胞
expressed in insect cells (S. frugiperda)
品質等級
化驗
≥95% (SDS-PAGE)
形狀
solid
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
雜質
<1.0 EU/μg protein Endotoxin
運輸包裝
ambient
儲存溫度
−20°C
一般說明
Dkk-2, Mouse, Recombinant, consists of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag sequence.
Recombinant, mouse Dickkopf related protein 2 (Dkk-2) consisting of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag sequence. Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations.
生化／生理作用
Inhibition assay: Inhibits WNT-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells in the presence of BMP-2.
Competetive Binding Assay:
Inhibits 50% binding of biotinylated rhDkk-1 (200 ng/ml) to immobilized rmKremen (4 µg/ml) at a concentration of 5-15 µg/ml.
Competetive Binding Assay:
Inhibits 50% binding of biotinylated rhDkk-1 (200 ng/ml) to immobilized rmKremen (4 µg/ml) at a concentration of 5-15 µg/ml.
警告
Toxicity: Standard Handling (A)
外觀
Lyophilized from 0.2 µm filtered PBS, BSA.
重構
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
其他說明
Morvan, F. et al. 2006. J. Bone Miner. Res.21, 934.
Mukhopadhyay, M. et al. 2006. Development133, 2149.
Niehrs, C. 2006. Oncogene25, 7469.
Li, X. et al. 2005. Nat. Genet.37, 945.
Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.
Mao, B. and C. Niehrs 2003. Gene302, 179.
Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol.22, 6100.
Li, L. et al. 2002. J. Biol. Chem.277, 5977.
Mao, B. et al. 2002. Nature417, 664.
Mao, B. et al. 2001. Nature411, 321.
Wu, W. et al. 2000. Current Biol.10,1611.
Monaghan, A.P. et al. 1999. Mech. Dev.87, 45.
Krupnik, V.E. et al. 1999. Gene238, 301.
Mukhopadhyay, M. et al. 2006. Development133, 2149.
Niehrs, C. 2006. Oncogene25, 7469.
Li, X. et al. 2005. Nat. Genet.37, 945.
Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.
Mao, B. and C. Niehrs 2003. Gene302, 179.
Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol.22, 6100.
Li, L. et al. 2002. J. Biol. Chem.277, 5977.
Mao, B. et al. 2002. Nature417, 664.
Mao, B. et al. 2001. Nature411, 321.
Wu, W. et al. 2000. Current Biol.10,1611.
Monaghan, A.P. et al. 1999. Mech. Dev.87, 45.
Krupnik, V.E. et al. 1999. Gene238, 301.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
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