219402

Cathepsin L, Human Liver

Cathepsin L, Human Liver, CAS 60616-82-2, is a native, the most potent of all the lysosomal proteinases. Plays a major role in the proteolysis of both cellular and endocytosed macromolecules.

• 别名: CTSL, CTSL1, Human Cathepsin L, Major excreted protein (MEP)
• EC 号: 3.4.22.15 (BRENDA, IUBMB)
• 分類程式碼代碼: 12352202
• NACRES: NA.77

属性

• 生物源: human liver
• 品質等級: 100
• 形狀: liquid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 技術: activity assay: suitable
• 適合性: suitable for molecular biology
• 應用: life science and biopharma
• 運輸包裝: wet ice
• 儲存溫度: −70°C
• 基因資訊: human ... CTSL(1514)

说明

一般說明

Research area: Cell Signaling
Cathepsin L, Human Liver, native, is the most potent of all the lysosomal proteinases. Cathepsin L (CTSL) belongs to the papain subfamily of cysteine proteases and is mainly located in endolysosomal vesicles.

生化／生理作用

Cathepsin L (CTSL) has higher activity than cathepsins B and H in the degradation of a variety of physiological protein substrates. It is believed to be responsible for the generation of endostatin from the NC1 domain in collagen XVII. It is responsible for regulating cell cycle, nucleotide-binding domain, leucine-rich–containing family, pyrin domain–containing-3 (NLRP3) inflammasome activation, proteolytic processing of Bid during apoptosis, and TGF-β signaling. It can degrade a wide range of proteins, encompassing enzymes, receptors, and transcription factors. Additionally, it produces active enzymes, receptors, and biologically active peptides through controlled proteolysis. Moreover, Cathepsin L has been observed to enhance tumor cell migration by lowering cell-cell adhesion and breaking down elements of the extracellular matrix. The expression of CTSL escalates in several types of cancers, including glioma, melanoma, pancreatic, prostate, and breast cancer. It plays a major role in the proteolysis of both cellular and endocytosed macromolecules.

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Z-FR-AFC per min at 25°C, pH 5.5.

外觀

In 400 mM NaCl, 20 mM malonate buffer, 1 mM EDTA, pH 5.5.

準備報告

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

重構

Following initial thaw, aliquot and freeze (-70°C).

其他說明

Note: 1 mU = 1 milliunit

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2