推荐产品
生物源
rabbit
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
rat (07-350SP, 07-363SP, 07-681SP), mouse (07-350SP), human (07-350SP, 07-363SP, 07-681SP)
製造商／商標名
Upstate®
濃度
1 mg/mL
技術
activity assay: suitable (kinase and phosphatase)
western blot: suitable
UniProt登錄號
運輸包裝
dry ice
基因資訊
human ... PRKAA1(5562)
mouse ... Hsf3(245525)
一般說明
Pathway Explorer Antibody MiniPack:
Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).
AMPK:
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
*See full size versions for corresponding references.
Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).
AMPK:
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
*See full size versions for corresponding references.
特異性
07-681SP: AMPKα phosphorylated on Thr172.
07-363SP: AMP-Activated Protein Kinase alpha 2 (AMPKα2).
07-350SP: AMPKα1
07-363SP: AMP-Activated Protein Kinase alpha 2 (AMPKα2).
07-350SP: AMPKα1
07-681SP: Expected to cross-react with mouse based on 100% sequence homology.
Cross Reactivty
Varies. See individual Data Sheets
Varies. See individual Data Sheets
免疫原
Epitope: 07-350SP and 07-363SP: Internal
應用
Research Category
Signaling
Apoptosis & Cancer
Signaling
Apoptosis & Cancer
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
This Antibody pack contains Anti-AMPK α1 Antibody, Anti-AMPKα2 Antibody, Anti-phospho-AMPKα Antibody(Thr172).
包裝
Each vial is 30% the size of the parent catalog number
成分
07-350SP Anti-AMPK α1; 60 µg
07-363SP Anti-AMPKα2; 60 µg
07-681SP Anti-phospho-AMPKα (Thr172); 60 µg
07-363SP Anti-AMPKα2; 60 µg
07-681SP Anti-phospho-AMPKα (Thr172); 60 µg
品質
07-681SP:Routinely evaluated by immunoblot on RIPA lysates from overnight starved HEK293 cells and partially purified rat liver AMPK.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected phospho-AMPK α in RIPA lysates from overnight serum starved HEK293 cells and partially purified rat liver AMPK. Signal was reduced after treatment of lysate with λ-phosphatase.
07-363SP: Routinely evaluated by immunoblot in a rat liver preparation.
Western Blot Analysis:
1-2 μg/mL of this lot detected AMPK α2 in a rat liver preparation.
07-350SP: Routinely evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected phospho-AMPK α in RIPA lysates from overnight serum starved HEK293 cells and partially purified rat liver AMPK. Signal was reduced after treatment of lysate with λ-phosphatase.
07-363SP: Routinely evaluated by immunoblot in a rat liver preparation.
Western Blot Analysis:
1-2 μg/mL of this lot detected AMPK α2 in a rat liver preparation.
07-350SP: Routinely evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.
標靶描述
~63 kDa;
外觀
Protein A purified
3 individual tubes containing either Anti-AMPK α1; Anti-AMPKα2; or Anti-phospho-AMPKα (Thr172)
Format: Purified
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
分析報告
Control
For 07-681SP use HEK293 cells or rat liver AMPK.
For 07-363SP use Rat liver preparation.
For 07-350SP use A431cell lysate, or lysate of HeLa cells treated with heat shock.
For 07-681SP use HEK293 cells or rat liver AMPK.
For 07-363SP use Rat liver preparation.
For 07-350SP use A431cell lysate, or lysate of HeLa cells treated with heat shock.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
The influence of carbohydrate-protein co-ingestion following endurance exercise on myofibrillar and mitochondrial protein synthesis.
Leigh Breen,Andrew Philp,Oliver C Witard,Sarah R Jackman,Anna Selby,Ken Smith,Keith Baar et al.
The Journal of Physiology null
Yiran Cui et al.
International journal of molecular sciences, 18(7) (2017-07-05)
Stroke is the second most common cause of death worldwide. A systematic description and characterization of the strokes and the effects induced in the hippocampus have not been performed so far. Here, we analysed the protein expression in the hippocampus
Nikolaos Nikolaou et al.
Metabolism: clinical and experimental, 99, 67-80 (2019-07-23)
Non-alcoholic fatty liver disease (NAFLD) is the hepatic manifestation of metabolic syndrome. Steroid hormones and bile acids are potent regulators of hepatic carbohydrate and lipid metabolism. Steroid 5β-reductase (AKR1D1) is highly expressed in human liver where it inactivates steroid hormones
Claudia Tonini et al.
Nutrients, 13(1) (2021-01-21)
A major problem of aging is the disruption of metabolic homeostasis. This is particularly relevant in the brain where it provokes neurodegeneration. Caloric restriction is a physiologic intervention known to delay the deleterious consequences of aging in several species ranging
Zoe E Gillespie et al.
Scientific reports, 9(1), 5369-5369 (2019-03-31)
Metformin is a widely-used treatment for type 2 diabetes and is reported to extend health and lifespan as a caloric restriction (CR) mimetic. Although the benefits of metformin are well documented, the impact of this compound on the function and
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