1.15934

Eosin B (bluish) (C.I.45400)

for microscopy Certistain^®

• 别名: Eosin B (bluish) (C.I.45400), Dibromodinitrofluorescein sodium
• 经验公式（希尔记法）: C₂₀H₆Br₂N₂Na₂O₉
• CAS号: 548-24-3
• 分子量: 624.06
• MDL號碼: MFCD00005041
• 分類程式碼代碼: 41116130
• 酶委員會索引編號: 208-943-1

属性

• 品質等級: 400
• 形狀: solid
• 成份: Dye, ≥85% spectrophotometric assay
• pH值: 6.2 (25 °C, 10 g/L in H₂O)
• 溶解度: 300 g/L
• 體積密度: 680 kg/m³
• εmax: 800-1000 at 517-525 nm in water at 0.005 g/L
• 應用: diagnostic assay manufacturing; hematology; histology
• 儲存溫度: 2-30°C
• SMILES 字串: [Na+].[Na+].Brc1c2c(cc(c1[O-])[N+](=O)[O-])C(=C4C(=C(C(=O)C(=C4)[N+](=O)[O-])Br)O2)c3c(cccc3)C(=O)[O-]
• InChI: 1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
• InChI 密鑰: GYYTYUGGVBYJHE-UHFFFAOYSA-L

说明

一般說明

Eosin B (C.I. 45400) for microscopy Certistain^®, is a dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin B staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear dark blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances pink to red. As the dye belongs to the portfolio of Certistain^® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

分析報告

Dye content (gravimetrically): ≥ 85 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 517 - 525 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; water): 800 - 1000
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test

法律資訊

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°C）: Not applicable