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Merck
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Key Documents

1.04242

Sigma-Aldrich

品红(C.I.42510)

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About This Item

经验公式(希尔记法):
C20H20ClN3
CAS号:
分子量:
337.85
分類程式碼代碼:
41116130
NACRES:
NA.21

形狀

solid

品質等級

成份

Dye, ≥80% spectrophotometric assay

pH值

5-6 (25 °C, 1 g/L in H2O)

mp

235 °C (decomposition)

溶解度

4 g/L

體積密度

500 kg/m3

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

2-30°C

InChI

1S/C20H19N3.ClH/c1-13-12-16(6-11-19(13)23)20(14-2-7-17(21)8-3-14)15-4-9-18(22)10-5-15;/h2-12,23H,21-22H2,1H3;1H

InChI 密鑰

AXDJCCTWPBKUKL-UHFFFAOYSA-N

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一般說明

This staining dye “Fuchsin (C.I. 42510) - for microscopy Certistain®” is used for staining of acid-fast bacteria (AFB) according to Kinyoun and serves the purpose of the bacteriological and histological investigation of sample material of human origin, for example smears of enriched bacterial cultures or histological sections of e. g. the lung. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. As a measure to enhance the absorption of the fuchsin dye and thus the formation of the mycolate-fuchsin complex in the cell wall, the carbolfuchsin solution applied to the specimen is normally heated to evaporation point. If the Kinyoun carbolfuchsin solution is used, the heating of the solution is dispensable. Consequently, the release of hazardous phenolic vapors will be avoided. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In the present operating instructions is counterstained accordance with methylene blue. Pretreatment of the specimens with Sputofluol dissolves the bacteria from the surrounding viscid sputum and cell material. As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.

應用

for microscopy Certistain®

分析報告

The dye has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

法律資訊

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

訊號詞

Danger

危險分類

Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3


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