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53829-U

Supelco

Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonyme(s) :

Core-shell (SPP) Fused Core C18 HPLC column

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About This Item

Code UNSPSC :
41115700
eCl@ss :
32110501
Nomenclature NACRES :
SB.52

product name

Colonne de CLHP Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

Matériaux

stainless steel column

Niveau de qualité

Agence

suitable for USP L1

Gamme de produits

Ascentis®

Caractéristiques

endcapped

Fabricant/nom de marque

Ascentis®

Conditionnement

1 ea of

Paramètres

60 °C temp. range
600 bar max. pressure (9000 psi)

Technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × D.I.

15 cm × 4.6 mm

Superficie

135 m2/g

Impuretés

<5 ppm metals

Matrice

Fused-Core particle platform
superficially porous particle

Groupe de la matrice active

C18 (octadecyl) phase

Taille des particules

2.7 μm

Dimension de pores

90 Å

pH de fonctionnement

2-9

Application(s)

food and beverages

Technique de séparation

reversed phase

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Description générale

Les colonnes de CLHP Ascentis Express, grâce à la technologie des particules Fused-Core®, vous permettent de bénéficier de la grande vitesse et de l′efficacité élevée des particules inférieures à 2 μm, tout en maintenant des contre-pressions plus faibles. L′association de la grande efficacité et des faibles contre-pressions est avantageuse pour les utilisateurs de systèmes UPLC® (ou d′autres systèmes à ultra-haute pression), ainsi qu′aux utilisateurs de la CLHP conventionnelle.
Rendez-vous sur la page d′accueil Ascentis Express pour obtenir plus d′informations sur cette nouvelle technologie de colonne.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

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Silvia Jakabová et al.
Journal of chromatography. A, 1232, 295-301 (2012-03-07)
Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a
Judy Stone
Methods in molecular biology (Clifton, N.J.), 1378, 301-320 (2015-11-26)
Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 %
Babu Rao Chandu et al.
SpringerPlus, 2(1), 194-194 (2013-06-07)
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human
Petra Šilarová et al.
Food chemistry, 237, 471-480 (2017-08-03)
The degradation of catechins and other phenolics in green tea infusions were monitored using fast HPLC/MS separation. The final separation was performed within 2.5min using Ascentis Express C18 column (50mm×2.1mm i.d.) packed with 2μm porous shell particles. Degradation was studied
Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within

Articles

Compendial methods from the USP (United States Pharmacopeia) are widely used in pharmaceutical drug product and raw materials testing. However, not all methods in the USP use modern technologies. In chromatographic methods, it is not uncommon that older brands of columns are specified. Therefore, the USP methods are under continuous revision to improve existing procedures or to allow the user to obtain better results.

Apixaban is sold under the tradename Eliquis and at present no pharmacopeial monographs are available for Axpixaban using either an assay and/or organic impurity profiling method. A newly developed and validated HPLC-UV method using a column with C18 solid core particles is demonstrated.

The HPLC method for ginsenoside separation was optimized using a ginsenoside standard mixture and then applied to a sample of American Ginseng root. The experimental conditions and chromatograms are shown.

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

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Protocoles

This paper illustrates the setting up of a Lopinavir assay following European pharmacopoeia guidelines (10.2), and using a shorter column packed with smaller particles. The described assay saves valuable time and improves separation efficiency, which typically translates to better method performance.

This method is particularly useful in research into the role of individual bile acids as signaling molecules; suitable for clinical laboratories to investigate potential mechanisms linked to gut hormone profiles and glycemic control.

HPLC Analysis of Benzene and Deuterated Benzene on Ascentis® Express C18

-methylcarbamate 10 μg/mL; Diuron; Propham; Siduron; Methiocarb, analytical standard; Linuron 10 μg/mL; Swep 10 μg/mL; Chlorpropham 10 μg/mL; Barban; Neburon

Contenu apparenté

Bile Acids (BA) are synthesized in the liver and play important roles in cholesterol homeostasis, absorption of vitamins and lipids, and various key metabolic processes.

Chromatograms

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Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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