T9285

Tampon Tris-EDTA

100X concentrate for Northern and Southern blotting

• Synonyme(s) : Tampon TE solution
• Numéro MDL: MFCD00236359
• Code UNSPSC : 41105319
• ID de substance PubChem : 329826904
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: for molecular biology
• Niveau de qualité: 200
• Stérilité: 0.2 μm filtered
• Forme: liquid
• pH: 7.8-8.2 (1 × in water)
• Application(s): sample preparation
• Activité étrangère: DNase, RNase, none detected
• Température de stockage: room temp
• Chaîne SMILES: NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
• Clé InChI: VLEIUWBSEKKKFX-UHFFFAOYSA-N

Description

Description générale

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Application

TE buffer has been used for:

• isolate, resuspend, or store DNA and RNA for PCR or qPCR
• gel electrophoresis, including Northern and Southern blotting
• bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
• antigen retrieval for immunohistochemistry

Composants

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Autres remarques

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Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable