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B6185

Sigma-Aldrich

Bionic™ Buffer, 10X Concentrate

for Northern and Southern blotting, solution

Synonyme(s) :

Sodium Borate Electrophoresis Buffer

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About This Item

Code UNSPSC :
41105319
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Niveau de qualité

200

Description

Mode of use Voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. User should test their chamber to determine optimum voltage.

Forme

solution

Concentration

10 ×

Impuretés

DNase, RNase and nickase, none detected

pH

8.0-8.5 (20-25 °C)

Température de stockage

room temp

Description générale

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

Application

Suitable for:
  • Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
  • Use with TBA and TAE gels
  • Preparation of agarose gels

Caractéristiques et avantages

  • Faster run times
  • Sharper bands
  • Easy to dilute and use
  • Flexible use with a variety of gel types

Conditionnement

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

Attention

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

Informations légales

Bionic is a trademark of Sigma-Aldrich Co. LLC

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H319,H360FD

Classification des risques

Eye Irrit. 2 - Repr. 1B

Code de la classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Carmen Naturil-Alfonso et al.
PloS one, 7(12), e51271-e51271 (2012-12-20)
Parthenogenetic embryos are one attractive alternative as a source of embryonic stem cells, although many aspects related to the biology of parthenogenetic embryos and parthenogenetically derived cell lines still need to be elucidated. The present work was conducted to investigate
Fast short-fragment PCR for rapid and sensitive detection of shrimp viruses.
Mrotzek G, et al.
Journal of Virological Methods, 168, 262-266 (2010)
Maria Desemparats Saenz-de-Juano et al.
Biology of reproduction, 87(4), 91-91 (2012-08-03)
Studies of embryo cryopreservation efficiency have focused mainly on technical and embryo factors. To determine how a slow freezing process affects embryo and fetal development, we studied in vivo development ability after the freezing procedure by assessing blastocyst development at

Protocoles

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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