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C7594

Sigma-Aldrich

Casein Blocking Buffer

for Northern and Southern blotting, powder blend

Synonyme(s) :

blocking buffer

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About This Item

Numéro CE :
232-555-1
Code UNSPSC :
12161700
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Forme

powder blend

Technique(s)

Southern blotting: suitable

pH

6.8-7.2(reconstituted per directions)

Solubilité

H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)

Température de stockage

room temp

Catégories apparentées

Description générale

Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.

Application

Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.

Caractéristiques et avantages

  • Better blocking performance than dried milk or BSA
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays

Notes préparatoires

Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

P7059

Solution tampon phosphate, 10× PBS for Western blots and IP

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

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Denhardt′s Solution, for Northern and Southern blotting, lyophilized powder

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P1415

Prehybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

85639

SSC Buffer, for Northern and Southern blotting, powder blend

93017

SSC Buffer 20x, for Northern and Southern blotting, solution

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

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Tadatsura Koshika et al.
Immunology, 134(4), 386-397 (2011-11-03)
α1,3-Galactosyltransferase gene-knockout pigs transgenic for porcine cytotoxic T-lymphocyte antigen 4 immunoglobulin (pCTLA4-Ig) have been produced to reduce T-cell-mediated rejection following xenotransplantation. The level of soluble pCTLA4-Ig in their blood was greatly in excess of the therapeutic level in patients, rendering
W Querbes et al.
Journal of virology, 80(19), 9402-9413 (2006-09-16)
Invasion of glial cells by the human polyomavirus, JC virus (JCV), leads to a rapidly progressing and uniformly fatal demyelinating disease known as progressive multifocal leukoencephalopathy. The endocytic trafficking steps used by JCV to invade cells and initiate infection are
Seiichi Katayama et al.
Acta medica Okayama, 60(6), 351-355 (2006-12-27)
Clostridium perfringens is an anaerobic spore-forming pathogen of humans and animals. C. perfringens type A strains, 13, CPN50, and NCTC8237, isolated from human gas gangrene, bound specifically to human fi bronectin (Fn). The trypsin-treatment of the bacterial cells significantly reduced
Xiaoli Ma et al.
BMC biochemistry, 11, 37-37 (2010-09-24)
Minichromosome maintenance proteins (Mcm) 2, 3, 4, 5, 6 and 7 are related by sequence and form a variety of complexes that unwind DNA, including Mcm4/6/7. A Mcm4/6/7 trimer forms one half of the Mcm2-7 hexameric ring and can be
Ochratoxin A in some French wines: application of a direct competitive ELISA based on an OTA-HRP conjugate.
Radoi, A., et al.
Analytical Letters, 42, 1187-1187 (2009)
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Protocoles

Casein Blocking Buffer Protocol for Southern or Western Blotting

Protocol for using Casein Blocking Buffer in Southern and Western blotting applications, including colorimetric and chemiluminescent substrate detection options. Troubleshooting for optimizing signal and reducing background is also included.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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