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T1706

Sigma-Aldrich

TransIT-CRISPR®

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.51

Application(s)

CRISPR

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Accomplish highly effective transfection of CRISPR/Cas9 DNA, RNA, Cas9-gRNA RNP (ribonucleoprotein) complexes and siRNA to knock-out, modify or knock-down genes of interest in a wide variety of cell types.

Caractéristiques et avantages

  • Highly efficient, non-liposomal polymeric transfection/delivery method for use with MISSION CRISPR/Cas9 DNA, RNA, Cas9 RNP Complexes and siRNA.
  • TransIT-CRISPR is a simple, fast, effective, and versatile. One transfection reagent to deliver CRISPR-Cas9 components in a variety of formats.
  • Effective in most cell lines including difficult-to-transfect cells.

Composants

0.4ml and 1ml DNA and siRNA non-liposomal, polymeric transfection reagent

Liaison

To order MISSION® CRISPR reagents, please click here

For more information and transfection protocols, please visit SigmaAldrich.com/TransITCRISPR

Autres remarques

  • TransIT-CRISPR has been tested in variety of cell types including U20S and HEK 293, A549 and HeLa among others.
  • It is recommended to transfect MISSION CRISPR DNA and RNA with the TransIT-CRISPR transfection reagent.
  • Sigma-Aldrich is the exclusive distributor of TransIT-CRISPR from Mirus Bio LLC.

Informations légales

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TransIT-CRISPR is a registered trademark of Mirus Bio LLC

Pictogrammes

FlameExclamation mark

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Irrit. 2 - Flam. Liq. 2

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

57.2 °F - closed cup

Point d'éclair (°C)

14.0 °C - closed cup


Certificats d'analyse (COA)

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Protocoles

Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity

Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

Contenu apparenté

The largest offering of quality Cas9 proteins ensures you have the perfect CRISPR reagents for your specific experimental needs. Rigorous purification, NLS signals, increased specificity and activity ensure your gene editing experiments are efficient and simple.

Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).

Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

Sigma-Aldrich® Advanced Genomics est le premier fournisseur de technologies d'édition et d'extinction géniques, dont CRISPR, Cas9, les ARN guides synthétiques (ARNsg) et les nucléases à doigts de zinc (ZFN pour "Zinc Finger Nucleases").

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