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DCAS9PROT

Sigma-Aldrich

dCas9-3XFLAG™-Biotin Protein

from Streptococcus pyogenes with D10A and H840A mutations, recombinant, expressed in E. coli, 1X NLS

Synonyme(s) :

Cas9 D10A H840A, Dead Cas9, dCas9, catalytically inactive Cas9

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.51

Produit recombinant

expressed in E. coli

Niveau de qualité

200

Pureté

≥90% (SDS-PAGE)

Forme

lyophilized powder

Conditionnement

pkg of 1 kit (3 components)

Conditions d'expédition

ambient

Température de stockage

−20°C

Description générale

Recombinant dCas9-3X FLAG-Biotin protein from Streptococcus pyogenes (~165 KD) is a ready-to-use reagent for genome engineering and genomic DNA detection, isolation and imaging experiments. When combined with target-specific guide RNAs, dead Streptococcus pyogenes Cas9 protein acts as a DNA targeting protein suitable for transfection of cell cultures, microinjection, DNA pull-down, chromatin enrichment, microbial identification and in situ DNA imaging. At the C terminus, the protein is tagged with a SV40 large T antigen nuclear localization signal (NLS) for nuclear import and both a 3XFLAG epitope and biotin for anti-FLAG®-based or streptavidin-based experiments.

Application

Functional Genomics/Target Validation/Genome Editing/Molecular Biology/Synthetic Biology/DNA detection/isolation/DNA imaging

Caractéristiques et avantages

  • Highly specific
  • Suitable for genomic DNA detection/isolation
  • Ready-to-inject/transfect

Conditionnement

  • pkg of 50 μg (≥ 300 pmol)
  • pkg of 250 μg (≥ 1500pmol)

Composants

Each kit consists of:
  • one vial of dCas9 recombinant protein
  • one vial containing 1 mL of 1× dilution buffer
  • one vial containing 1 mL of Nuclease free water with glycerol

Principe

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be rendered inactive (dCas9) with mutations to the two protein domains, RuvC and HnH (D10A and H840A respectively), which are responsible for nuclease activity. The protein can then be programmed with a gRNA and directed to bind at a desired sequence of DNA.

Reconstitution

Lyophilized dCas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.

Autres remarques

Use our CRISPR Selection Tool to order gRNA

Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins

Informations légales

CRISPR Use License Agreement
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Protocoles

PURedit™ CRISPR Synthetic Guide RNAs and Cas9 Protein

Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity

Ribonucleoprotein (RNP) Protocols Using Synthetic sgRNAs and Cas9 proteins

Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

Contenu apparenté

CRISPR Cas9 Ribonucleoprotein (RNP) Application Note

Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

Visualize your CRISPR Experiment with MISSION

Watch Cas9-GFP proteins work inside your cells and deliver the powerful nuclease editing you have come to trust and expect. MISSION™ SpCas9-GFP and eSpCas9-GFP proteins are multi-functional enzymes with high on-target cleavage efficiency fused to Enhanced Green Fluorescent Protein (EGFP) for real-time observation of Cas9 delivery and clearance from cells.

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