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FNCAS9TRACR

Sigma-Aldrich

SygRNA™ FnCas9 Synthetic tracrRNA

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About This Item

Code UNSPSC :
41106609
Nomenclature NACRES :
NA.51

Conditionnement

pkg of 1 vial

Conditions d'expédition

ambient

Température de stockage

−20°C

Description générale

The SygRNA synthetic RNA system recruits FnCas9 nuclease to specific double stranded DNA targets. The FnCas9 tracrRNA (trans-activating crRNA) binds to a FnCas9 crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides FnCas9 protein to crRNA-specified sites in double stranded DNA. SygRNA FnCas9 synthetic tracrRNA and crRNAs are optimized for use with FnCas9 protein and compatible with a variety of nucleic acid delivery and digestion methods. To purchase custom crRNAs click "Request Quotation for Custom crRNA Synthesis" at sigma.com/SygRNA

Application

Functional Genomics/Genome Editing/Molecular Biology/Molecular Cloning/Synthetic Biology

Caractéristiques et avantages

HPLC-purified SygRNA FnCas9 tracrRNA oligo is optimized for use with target-specific Francisella novicida Cas9 crRNA and FnCas9 protein for genome editing applications, large fragment molecular cloning, and programmable restriction digestion.

Forme physique

Dry

Notes préparatoires

Resuspend SygRNA FnCas9 tracrRNA oligo in RNase-free Tris-containing buffer with a pH 8.0 or less.

Informations légales

CRISPR Use License Agreement
SygRNA is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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