Accéder au contenu
Merck
Toutes les photos(1)

Principaux documents

Voir toute la documentation

SRE0066

Sigma-Aldrich

Guanidine hydrochloride solution, 6M

Synonyme(s) :

Aminoformamidine hydrochloride, GuHCl, Guanidinium chloride, Guanidium chloride

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352209
Nomenclature NACRES :
NA.25

Forme

liquid

Niveau de qualité

500

Concentration

6 M

Application(s)

diagnostic assay manufacturing

Température de stockage

room temp

Catégories apparentées

Application

Guanidine hydrochloride is commonly used as a denaturant, because of its ability to break hydrogen bonds between amino acid residues. By breaking these bonds, the 3D conformation of the protein is unfolded and the aqueous solubility of the protein is greatly increased. Once denatured, the protein can be easily reduced, modified, or analyzed, in a variety of procedures. Further processing of the sample can allow the proteins to be analyzed by other common protein analysis methods including mass spectrometry, electrophoresis, and enzymatic digests.
Guanidine hydrochloride has also typically been used for the isolation of RNA, to denature globular proteins, and for protein refolding studies. It can also be used to facilitate the generation of tryptic peptides for analysis of complex protein samples.

Forme physique

This product is a ready-to-use 6 M guanidine hydrochloride solution. The 6 M Guanidine hydrochloride solution may be used as a stock solution to prepare solutions of lower concentrations as appropriate.

Note: If precipitation is present in the 6 M buffer, warm the bottle to 37 °C and mix until completely dissolved prior to use.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H302,H315,H319

Classification des risques

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
SLCK1591
SLCJ3806
SLCL5813
SLCL3308
SLCK8579

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Kaamini M Dhanabalan et al.
Biomaterials science, 8(15), 4308-4321 (2020-07-01)
Osteoarthritis (OA) is a joint disease characterized by progressive damage of articular cartilage and the adjoining subchondral bone. Chondrocytes, the primary cells of the cartilage, have limited regenerative capacity and when they undergo stress due to trauma or with aging
Yoshinori Aso et al.
eLife, 8 (2019-11-15)
Animals employ diverse learning rules and synaptic plasticity dynamics to record temporal and statistical information about the world. However, the molecular mechanisms underlying this diversity are poorly understood. The anatomically defined compartments of the insect mushroom body function as parallel

Articles

Workflow for the Analysis of Polysorbate 80 in Erbitux® Formulation

A complete reversed phase HPLC-ELSD workflow, including sample preparation for the analysis of Polysorbate 80 in a monoclonal antibody formulation.

Protocoles

Workflow for High-Throughput Glycoprofiling of Rituximab

Step-by-step SEC-MS protocol for rapid glycoprofiling of a monoclonal antibody (mAb), consisting of antibody purification, a mAb reduction procedure, a mass spectrometer calibration, and a system suitability test.

Protocol for Purification, Optional Reduction, and SEC-MS Analysis of a Monoclonal Antibody

A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.

Contenu apparenté

Middle-up Mass Analysis of Protease Digested Cetuximab

Step-by-step reversed phase UHPLC-MS workflow for middle-up mass analysis of an immunoglobulin G antibody, consisting of antibody purification, IdeS proteolysis and reduction, mass spectrometer calibration, mAb quantification, and a system suitability test.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique