P3375
Papaïne from papaya latex
crude powder, 1.5-10 units/mg solid
Synonyme(s) :
Papaïnase
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Forme
crude powder
Niveau de qualité
Activité spécifique
1.5-10 units/mg solid
Poids mol.
23.4 kDa
Température de stockage
2-8°C
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Application
Papain from papaya latex has been used:
- in the enzyme cocktail to evaluate the efficacy of B complex vitamers liberation from nutritional yeast powder
- in the enzyme mixture for the digestion of bovine milk samples for the analysis of three vitamin B1 molecules
- as a biocatalyst for the hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P3375, papain crude powder, has been used to digest fresh cartilage of dogsfish in order to isolate chondroitin sulfate.
Sert à produire des fragments Fab d′anticorps. Également utilisée pour la dissociation cellulaire, car des études ont montré qu′elle s′avère plus efficace et moins détériorante avec certains tissus.
Actions biochimiques/physiologiques
Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Protéase à cystéine qui clive les liaisons peptidiques des acides aminés basiques, de la leucine ou de la glycine.
pH optimal situé entre 6,0 et 7,0.
Hydrolyse également les esters et les amides.
pH optimal situé entre 6,0 et 7,0.
Hydrolyse également les esters et les amides.
Qualité
Not standardized with lactose or other adulterants.
Définition de l'unité
One unit will hydrolyze one micromole of N-alpha-benzoyl-l-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.
Autres remarques
Pour en savoir plus sur la papaïne, rendez-vous sur www.sigma-aldrich.com/enzymeexplorer.
Inhibiteur
Réf. du produit
Description
Tarif
Substrat
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Determination of the native forms of vitamin B1 in bovine milk using a fast and simplified UHPLC method
Schmidt A, et al.
Food Chemistry, 229, 452-457 (2017)
Carica papaya lipase: a novel biocatalyst for the enantioselective hydrolysis of (R, S)-naproxen 2, 2, 2-trifluoroethyl ester
Chen CC and Tsai SW
Enzyme and Microbial Technology, 36(1), 127-132 (2005)
Comparison of different extraction methods for simultaneous determination of B complex vitamins in nutritional yeast using LC/MS-TOF and stable isotope dilution assay
Halvin K, et al.
Analytical and Bioanalytical Chemistry, 405(4), 1213-1222 (2013)
Valentina Gargiulo et al.
Glycobiology, 19(12), 1485-1491 (2009-08-22)
Chondroitin sulfate (CS), a constituent of proteoglycans, is a key component of the connective tissues and it is widely used as a precautionary drug for joint diseases; for this reason, the increased demand of this polysaccharide has posed the problem
Tarik Bozoglu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(7), e2103867-e2103867 (2022-01-14)
Adeno-associated viruses (AAVs) are frequently used for gene transfer and gene editing in vivo, except for endothelial cells, which are remarkably resistant to unmodified AAV-transduction. AAVs are retargeted here toward endothelial cells by coating with second-generation polyamidoamine dendrimers (G2) linked
Articles
Papain is a cysteine protease of the peptidase C1 family. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique