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MAK129

Sigma-Aldrich

β-Glucosidase Activity Assay Kit

sufficient for 100 colorimetric tests

Synonyme(s) :

Beta-Glucosidase Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

neurological disorders; gastrointestinal diseases; hematological disorder

Température de stockage

−20°C

Informations sur le gène

human ... GBA(2629) , GBA2(57704) , GBA3(57733)
mouse ... GBA(14466) , GBA2(230101)
rat ... GBA(684536) , GBA2(298399) , GBA3(289687)

Description générale

β-Glucosidase hydrolyzes carbohydrates by acting on terminal, non-reducing b(1→4)-linked D-glucose residues with the release of D-glucose. β-Glucosidases are required by organisms for the consumption of cellulose. Lysozyme, a β-glucosidase present in tears, acts on the β(1→4) glucose bonds present in the peptidoglycan cell wall of Gram-negative bacteria and helps to prevent bacterial infections in the eye. Defects in β-glucosidase activity have been implicated in Gaucher′s disease and Parkinson′s disease.

Application

β-Glucosidase Activity Assay Kit has been used to measure the β-glucosidase activity of the sample.

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

The β-Glucosidase Activity Assay kit provides a simple and direct procedure for measuring β-glucosidase activity in biological samples, for characterizing β-glucosidase production, and for the screening of β-glucosidase modulators.

Principe

In this assay, β-glucosidase activity is determined by a reaction in which β-glucosidase hydrolyzes p-nitrophenyl-β-D-glucopyranoside resulting in the formation of a colorimetric (405 nm) product, proportional to the β-glucosidase activity present. One unit of β-Glucosidse is the amount of enzyme that catalyzes the hydrolysis of 1.0 μmole substrate per minute at pH 7.0.

Code de la classe de stockage

12 - Non Combustible Liquids

Certificats d'analyse (COA)

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Spruce budworm (lepidoptera: tortricidae) oral secretions II: Chemistry.
Leclair G, et al.
Environmental Entomology, 44(6), 1531-1543 (2015)
Alexandre Henriques et al.
Scientific reports, 7(1), 5235-5235 (2017-07-14)
Recent metabolomic reports connect dysregulation of glycosphingolipids, particularly ceramide and glucosylceramide, to neurodegeneration and to motor unit dismantling in amyotrophic lateral sclerosis at late disease stage. We report here altered levels of gangliosides in the cerebrospinal fluid of amyotrophic lateral

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