GALS

β-Galactosidase Reporter Gene Staining Kit

• Synonyme(s) : β-galactosidase, LacZ
• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.32

Propriétés

• Utilisation: kit sufficient for 100 tests (using a 3.5 cm dish)
• Niveau de qualité: 200
• Technique(s): microbe id | staining: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

β-galactosidase (β-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of β-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of β-gal like O-nitrophenyl-β-D-galactopyranoside (ONPG). β-gal is a cost-effective, simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the β-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.

Application

β-Galactosidase Reporter Gene Staining Kit has been used for histochemical staining of animal cells or tissues such as:

• sagittal brain sections in histoenzymology
• pancreas or colon sections in β-galactosidase reporter gene assay
• mice E10.5 -11.5 embryo and embryo sections

Autres remarques

The substrate used in this kit is X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) which results in an indigo blue-colored staining of transfected cells and tissues. This test provides a method for determining the percentage of transfected cells that are expressing LacZ or for microscopically visualizing reporter gene expression in tissue sections.

Informations sur la sécurité

• Pictogrammes: GHS02,GHS06,GHS08,GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H226,H302 + H312,H314,H317,H331,H334,H335,H341,H350,H412
• Conseils de prudence: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Organes cibles: Respiratory system
• Risques supp: EUH032
• Code de la classe de stockage: 3 - Flammable liquids
• Point d'éclair (°F): 132.8 °F
• Point d'éclair (°C): 56 °C