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MAK009

Sigma-Aldrich

Amylase Activity Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

endocrinological disorders, diabetes; gastrointestinal diseases

Température de stockage

−20°C

Informations sur le gène

human ... AMY1A(278)

Description générale

The new Amylase Assay Kit, MAK478, is now available! Amylase enzymes are glycoside hydrolases, which cleave glucan linkages in polysaccharides such as starch or glycogen to release polysaccharide molecules. α-Amylase is found in animals, plants, and many fungi. In mammals, α-amylase is the predominant form of amylase and is one of the major enzymes of dietary digestion. Both the activity and the stability of α-amylase is dependent on calcium ions. In humans, α-amylase levels are highest in pancreatic and salivary secretions. Increased blood and urine levels of amylase occur due to pancreatic disorders and salivary trauma. Increased salivary amylase activity is also a marker for increased sympathetic nervous system activity.

Application

Amylase Activity Assay Kit has been used to determine the activity of amylase.

Adéquation

Suitable for the quantification of amylase activity in a variety of biological samples.

Principe

The Amylase Activity Assay kit quantifies amylase activity in a variety of biological samples. The assay is quick, convenient, and sensitive. In this kit, amylase activity is determined using a coupled enzymatic assay, which results in a colorimetric (405 nm) product, proportional to the amount of substrate, ethylidene-pNP-G7, cleaved by the amylase. One unit is the amount of amylase that cleaves ethylidene-pNP-G7 to generate 1.0 μmole of p-nitrophenol per minute at 25 °C.

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Description
Tarif

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Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Production, Biocatalysis and Industrial Applications
Goutam B.
Biotechnology of microbial enzymes (2016)
High endogenous salivary amylase activity is associated with improved glycemic homeostasis following starch ingestion in adults.
Mandel A L, et al.
The Journal of Nutrition, 142(5), 853-858 (2012)
Philip van der Zee et al.
Purinergic signalling (2018-05-13)
Recent research suggested an important role for pulmonary extracellular adenosine triphosphate (ATP) in the development of ventilation-induced lung injury. This injury is induced by mechanical deformation of alveolar epithelial cells, which in turn release ATP to the extracellular space. Measuring
Usefulness of salivary alpha amylase as a biomarker of chronic stress and stress related oral mucosal changes?a pilot study.
Vineetha R, et al.
Journal of Clinical and Experimental Dentistry, 6(2), e132-e132 (2014)
Natalie Chiu et al.
Food hydrocolloids, 69, 450-458 (2017-08-05)
Sodium (salt) was encapsulated within the inner water phase of w1/o/w2 food emulsions externally stabilised by starch particles with the ultimate aim of enhancing saltiness perception. The physical properties of the starch particles were modified by octenyl succinic anhydride (OSA)
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Articles

Enzymatic Method for Determining Amylase Activity (Amylase Activity Assay)

Assay protocol for the colorimetricdetection of amylase activity in biological samples using the Amylase Activity assay kit.

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