MAK009
Amylase Activity Assay Kit
sufficient for 100 colorimetric tests
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Utilisation
sufficient for 100 colorimetric tests
Méthode de détection
colorimetric
Maladie(s) pertinente(s)
endocrinological disorders, diabetes; gastrointestinal diseases
Température de stockage
−20°C
Informations sur le gène
human ... AMY1A(278)
Catégories apparentées
Description générale
The new Amylase Assay Kit, MAK478, is now available! Amylase enzymes are glycoside hydrolases, which cleave glucan linkages in polysaccharides such as starch or glycogen to release polysaccharide molecules. α-Amylase is found in animals, plants, and many fungi. In mammals, α-amylase is the predominant form of amylase and is one of the major enzymes of dietary digestion. Both the activity and the stability of α-amylase is dependent on calcium ions. In humans, α-amylase levels are highest in pancreatic and salivary secretions. Increased blood and urine levels of amylase occur due to pancreatic disorders and salivary trauma. Increased salivary amylase activity is also a marker for increased sympathetic nervous system activity.
Application
Amylase Activity Assay Kit has been used to determine the activity of amylase.
Adéquation
Suitable for the quantification of amylase activity in a variety of biological samples.
Principe
The Amylase Activity Assay kit quantifies amylase activity in a variety of biological samples. The assay is quick, convenient, and sensitive. In this kit, amylase activity is determined using a coupled enzymatic assay, which results in a colorimetric (405 nm) product, proportional to the amount of substrate, ethylidene-pNP-G7, cleaved by the amylase. One unit is the amount of amylase that cleaves ethylidene-pNP-G7 to generate 1.0 μmole of p-nitrophenol per minute at 25 °C.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
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