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MAK107

Sigma-Aldrich

LCAT Activity Assay Kit

Supplied by Roar Biomedical, Inc.

Synonyme(s) :

Lecithin:cholesterol acyltransferase activity assay kit

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About This Item

Numéro CE :
200-659-6
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 fluorometric tests

Application(s)

pharmaceutical

Méthode de détection

fluorometric

Maladie(s) pertinente(s)

cardiovascular diseases

Température de stockage

−20°C

Informations sur le gène

human ... LCAT(3931)

Description générale

The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol resulting in the formation cholesteryl ester. This enzymatic activity occurs on the surface of high density lipoprotein (HDL). The cholesteryl esters formed by LCAT may be packed into the core of HDL.

Application

Applications for this method include high-throughput screening, mechanism of action studies and structure-activity relationship (SAR) work.
LCAT Activity Assay Kit has been used for measuring lecithin-cholesterol acyl transferase activity in plasma samples.

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

Suitable for measuring phospholipase activity of LCAT in plasma or serum.

Principe

The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of (λEx=390 nm/λEm=470 nm) LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman′s reagent) LCAT inhibitor.

Remarque sur l'analyse

Learn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.

Composants de kit seuls

Réf. du produit
Description
  • Substrate Reagent .1 mL
  • READ Reagent 30 mL
  • LCAT Assay Buffer 20 mL

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

FlameSkull and crossbonesHealth hazard
GHS02,GHS06,GHS08

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

49.5 °F

Point d'éclair (°C)

9.7 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Protocoles

LCAT Activity Assay Kit

The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of LCAT.

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