F6008

Ficin from fig tree latex

lyophilized powder

• Synonyme(s) : Debricin, Ficain, higueroxyl delabarre
• Numéro CAS: 9001-33-6
• Numéro de classification (Commission des enzymes): 3.4.22.3 (BRENDA, IUBMB)
• Numéro CE : 232-599-1
• Numéro MDL: MFCD00081597
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: fig tree (latex)
• Niveau de qualité: 300
• Forme: lyophilized powder
• Activité spécifique: ≥1.0 units/mg protein
• Poids mol.: 23.8 kDa
• Composition: Protein, 80-100% biuret
• Température de stockage: −20°C

Description

Description générale

Extinction Coefficient: E^1% = 21.0 (280 nm)
pI: 9.0

Application

Ficin from fig tree latex has been used:

• in the proteolysis of tendon collagen
• in the digestion of Chlorophyta Ulva rigida
• to investigate it effect on protease-activated receptors 2 and 4 activation to induce itch in skin

Actions biochimiques/physiologiques

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported K_m for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)₂ fragments from mouse IgG1.

Ficin is effective in disrupting the staphylococcal biofilms making it accessible antibiotics exposure.

Définition de l'unité

One unit will produce a ΔA₂₈₀ of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).

Forme physique

Lyophilized powder containing L-cysteine and trace EDTA

Notes préparatoires

This enzyme is soluble in water (10 mg/ml), yielding a clear solution.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves