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F1269

Sigma-Aldrich

Anti-Avidin–FITC antibody, Mouse monoclonal

clone WC19.10, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-Avidin

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

mouse

Conjugué

FITC conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

WC19.10, monoclonal

Forme

buffered aqueous solution

Conditions de stockage

protect from light

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect immunofluorescence: suitable

Isotype

IgG1

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Monoclonal Anti-Avidin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Avidin is a glycoprotein (68 kDa) composed of four identical polypeptide chains isolated from egg whites.
The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin.

Spécificité

FITC conjugated monoclonal anti-avidin may be used for single or double immunofluorescence labeling techniques and for modification of non-fluorescent to fluorescent reactions.

Immunogène

Purified egg white avidin.

Application

Anti-Avidin-FITC antibody, Mouse monoclonal has been used in indirect immunohistology.

Actions biochimiques/physiologiques

Avidin has a high binding affinity for the vitamin biotin. This strong interaction has been exploited in the design of immunoassays and immunohistologic staining techniques to serve as a basis for identifying antigen antibody interactions

Forme physique

Solution supplied in 0.01 M phosphate buffered saline, pH 8.0, containing 1% BSA and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Adsorption of avidin on microfabricated surfaces for protein biochip applications
Bashir R, et al.
Biotechnology and Bioengineering, 73(4), 324-328 (2001)
Sergey Mursalimov et al.
Protoplasma, 254(3), 1379-1384 (2016-09-30)
Cytomixis is a poorly studied process of nuclear migration between plant cells, discovered in microsporogenesis of several hundreds of plant species. The chromosomes that migrate between tobacco microsporocytes have been for the first time identified using fluorescence in situ hybridization
Xuefeng Wang et al.
Applied optics, 47(15), 2779-2789 (2008-05-22)
We perform simultaneous interferometric and fluorescent detection of molecular protein layers on a BioCD. The 488 nm excitation wavelength of fluorescein also provides the interferometric detection channel that operates in a common-path in-line configuration in the condition of phase quadrature

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