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Key Documents

A8706

Sigma-Aldrich

Avidin from egg white

recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)

Synonyme(s) :

AVID_CHICK

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About This Item

Numéro CAS:
1405-69-2
Numéro CE :
215-783-6
Numéro MDL:
MFCD00130572
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.56

Produit recombinant

expressed in corn

Pureté

≥80% protein basis (biuret)

Forme

powder

Activité spécifique

≥12 units/mg protein (E1%/280)

Poids mol.

glycoprotein 66 kDa
subunit 16 kDa

Technique(s)

ELISA: suitable
immunoblotting: suitable
immunoelectrophoresis: suitable

Numéro d'accès UniProt

P02701

Température de stockage

2-8°C

Informations sur le gène

chicken ... AVD(396260)

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Description générale

Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.

Application

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.

Définition de l'unité

One unit will bind 1.0 μg of d-biotin.

Notes préparatoires

Affinity purified

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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M J Navakouskiĭ et al.
Bioorganicheskaia khimiia, 38(4), 449-457 (2012-11-30)
A new construction of a test-system for free thyroid hormone 3,3',5-triiodothyronine determination (free T3) in human blood serum in ELISA and IRMA variants was described. For this purpose a low-molecular weight bifunctional conjugate containing T3 and vitamin H (biotin, Bt)
Sofia H L Frost et al.
Cancer biotherapy & radiopharmaceuticals, 28(2), 108-114 (2012-12-13)
Abstract Purpose: Pretargeted radioimmunotherapy (PRIT) against intraperitoneal (i.p.) ovarian microtumors using avidin-conjugated monoclonal antibody MX35 (avidin-MX35) and (211)At-labeled, biotinylated, succinylated poly-l-lysine ((211)At-B-PLsuc) was compared with conventional radioimmunotherapy (RIT) using (211)At-labeled MX35 in a nude mouse model. Mice were inoculated i.p.
Stefan Schillberg et al.
Journal of plant physiology, 258-259, 153359-153359 (2021-01-19)
Recombinant proteins play an important role in many areas of our lives. For example, recombinant enzymes are used in the food and chemical industries and as high-quality proteins for research, diagnostic and therapeutic applications. The production of recombinant proteins is
Minkoo Ahn et al.
PloS one, 7(11), e50192-e50192 (2012-11-21)
Fibril formation by mutational variants of human lysozyme is associated with a fatal form of hereditary non-neuropathic systemic amyloidosis. Defining the mechanistic details of lysozyme aggregation is of crucial importance for understanding the origin and progression of this disease and
Biopharmaceuticals in Plants: Toward the Next Century of Medicine, 121-122 (2009)
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