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Key Documents

A2901

Sigma-Aldrich

Avidin–FITC from egg white

lyophilized powder

Synonyme(s) :

Avidin–Fluorescein isothiocyanate from egg white

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About This Item

Numéro MDL:
MFCD00130585
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Conjugué

FITC conjugate

Niveau de qualité

200

Forme

lyophilized powder

Ampleur du marquage

2-4 mol fluorescein isothiocyanate per mol avidin

Technique(s)

immunohistochemistry: suitable

Température de stockage

−20°C

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Description générale

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin-FITC from egg white has been used for immunohistochemistry applications.
To determine the amount of labeled polyclonal IgG present in spores in an IgG prep. A known concentration of FITC avidin from egg white and biotin-IgG avidin was used in excess at 300nm.

Conditionnement

Package size based on protein content

Forme physique

Contains sodium citrate

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

J C Sørensen et al.
Experimental brain research, 112(2), 203-212 (1996-11-01)
Thalamic neuronal degeneration after neocortical lesions involve both anterograde and retrograde components. This study deals with the thalamic microglial response after neocortical aspiration lesions, using fluorogold fluorescent prelabeling, to identify retrogradely degenerating thalamocortical neurons, combined with histochemical or immunohistochemical staining
Lizanne G Nilewski et al.
ACS chemical neuroscience, 9(1), 107-117 (2017-07-29)
Selective targeting of drug loaded nanovectors to specific epitopes highly expressed on the surface of cancer cells is a goal for nanotechnologists. We have modified our previously described PEGylated-hydrophilic carbon clusters (PEG-HCCs) so that the epidermal growth factor receptor (EGFR)
Sergey Mursalimov et al.
Protoplasma, 254(3), 1379-1384 (2016-09-30)
Cytomixis is a poorly studied process of nuclear migration between plant cells, discovered in microsporogenesis of several hundreds of plant species. The chromosomes that migrate between tobacco microsporocytes have been for the first time identified using fluorescence in situ hybridization
S M Hsu et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 29(4), 577-580 (1981-04-01)
The use of avidin-biotin interaction in immunoenzymatic techniques provides a simple and sensitive method to localize antigens in formalin-fixed tissues. Among the several staining procedures available, the ABC method, which involves an application of biotin-labeled secondary antibody followed by the

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