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CMC0001

Sigma-Aldrich

SIG10 Chemically Competent Cells

Escherichia coli, rod shaped

Synonyme(s) :

Chemically competent E. coli, Competent bacterial cells

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About This Item

Code UNSPSC :
41106202
Nomenclature NACRES :
NA.85

product name

SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production

Source biologique

Escherichia coli

Qualité

for molecular biology

Forme

buffered aqueous solution

Mode de croissance

adherent or suspension

Morphologie

rod shaped

Technique(s)

microbiological culture: suitable

Transformation cellulaire

competent cell type: chemically competent
transformation efficiency: ≥1 × 109 cfu/μg

Conditions d'expédition

dry ice

Température de stockage

−70°C

Catégories apparentées

Description générale

The SIG10 Chemically Competent Cells are a derivative of Escherichia coli that have been optimized for high transformation efficiency.
These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones and are highly efficient for routine cloning applications.
They share the most useful genetic elements of standard cloning strains like DH5α™ DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
They are are provided in 40 μL, 80 μL and 160 μL aliquots, each being sufficient for one, two and four transformations respectively.
The cells have a transformation efficiency of >1 x 109 cfu/μg
The 96-well format are provided in aliquots of 20 μL per well and have a transformation efficiency of >1 x 108 cfu/μg.

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA

Application

Suitable for bacterial transformations to recover high quality plasmid DNA

Caractéristiques et avantages

  • share the most useful genetic elements of standard cloning strains like DH5α™ DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
  • ensures recovery of stable and high quality plasmid DNA.
  • high transformation efficiency
  • provide the performance researchers need with ease of use.
  • provide solutions for a wide range of applications at economical prices.
  • Blue - white screening

Composants

  • SIG10 chemically competent cells
  • pUC 19 transformation control DNA at 10 pg/μL
  • recovery medium for cloning

Principe

The SIG10 cells contains the inactive mcr and mrr alleles, allowing methylated genomic DNA isolated directly from mammalian or plant cells to be cloned without deletions or rearrangements.
This strain also carries the recA1 and endA1 mutations. The recA1 genotype provides minimized recombination and aids in plasmid stability while endA1 provides for high quality plasmid DNA preparation.
They are bacteriophage T1-resistant (tonA mutation) and also resistant to streptomycin by virtue of rpsL mutation.

Informations légales

DH5α is a trademark of Invitrogen Corp.

Code de la classe de stockage

10 - Combustible liquids

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Articles

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Bacterial Transformation

Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.

Protocoles

SIG10 Chemically Competent Cells

For best results, ligation reactions must be heat inactivated at 70º C for 15 minutes before transformation. Alternately, the reactions may be purified.

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