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Key Documents

CMC0014

Sigma-Aldrich

BL21(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

Synonyme(s) :

Competent E. coli cells

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About This Item

Code UNSPSC :
41106202
Nomenclature NACRES :
NA.85

product name

BL21(DE3) Chemically Competent Cells, for protein expression

Source biologique

Escherichia coli

Qualité

for molecular biology

Mode de croissance

adherent or suspension

Morphologie

rod shaped

Technique(s)

microbiological culture: suitable

Transformation cellulaire

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

Conditions d'expédition

dry ice

Température de stockage

−70°C

Description générale

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Application

The E. coli strain BL21 (DE3) has been used:

  • to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.
  • BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.
  • to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.

Caractéristiques et avantages

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

Composants

  • BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

L3022

Bouillon LB (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

L2897

Bouillon LB avec gélose (Lennox), Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

B11001

EnPresso® B, Growth system for expressing protein in bacteria

H9950

Hanahan′s Broth (SOB Medium), Liquid microbial growth medium

CMC0015

BL21(DE3) pLysE Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

CMC0023

CONTROLLER SIG10 Chemically Competent Cells, for controlled protein expression

CMC0024

CONTROLLER BL21(DE3) Chemically Competent Cells, for controlled protein expression

CMR0001

Recovery Medium for Expression, for use with competent cells

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Yasaman Damestani et al.
Lasers in surgery and medicine, 48(8), 782-789 (2016-10-19)
The development and feasibility of a novel nanocrystalline yttria-stabilized-zirconia (nc-YSZ) cranial implant has been recently established. The purpose of what we now call "window to the brain (WttB)" implant (or platform), is to improve patient care by providing a technique
A validated antibody panel for the characterization of tau post-translational modifications.
Ebru E, et al.
Mol. Neurodegener., 12(1), 87-87 (2017)
Romana Koszagova et al.
Microbial cell factories, 17(1), 139-139 (2018-09-05)
Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction
David B Sauer et al.
eLife, 9 (2020-09-02)
Citrate, α-ketoglutarate and succinate are TCA cycle intermediates that also play essential roles in metabolic signaling and cellular regulation. These di- and tricarboxylates are imported into the cell by the divalent anion sodium symporter (DASS) family of plasma membrane transporters
Gints Kalnins et al.
Nature communications, 11(1), 388-388 (2020-01-22)
Bacterial microcompartments (BMCs) are prokaryotic organelles consisting of a protein shell and an encapsulated enzymatic core. BMCs are involved in several biochemical processes, such as choline, glycerol and ethanolamine degradation and carbon fixation. Since non-native enzymes can also be encapsulated
Afficher tous les articles scientifiques apparentés

Articles

Bacterial Transformation

Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.

Contenu apparenté

BL21 Chemically Competent Cells

BL21 Chemically Competent Cells are provided in aliquots of 80 μL sufficient for two transformation reactions of 40 μL each. Transformation is performed by heat shock at 42 °C, followed by incubation on ice.

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