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69825-M

Sigma-Aldrich

NovaBlue Competent Cells - Novagen

Escherichia coli, rod shaped

Synonyme(s) :

Competent Cells for Transformation

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About This Item

Code UNSPSC :
41106202
Nomenclature NACRES :
NA.81

product name

NovaBlue Competent Cells - Novagen, NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.

Source biologique

Escherichia coli

Niveau de qualité

Fabricant/nom de marque

Novagen®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Mode de croissance

adherent or suspension

Morphologie

rod shaped

Technique(s)

microbiological culture: suitable

Transformation cellulaire

transformation efficiency: >1×108 cfu/μg

Conditions d'expédition

dry ice

Température de stockage

−70°C

Description générale

NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations, which result in high yields of excellent quality plasmid DNA. The DE3 lysogen of NovaBlue is potentially useful as a stringent host due to the presence of the lacIq repressor encoded by the F episome. Blue/white screening is not possible with NovaBlue(DE3) due to the presence of the lacZ α-peptide coding sequences in the lysogenic phage.
NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Composants

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlNovaBlue Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

Avertissement

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Informations légales

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2


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