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A1960

Sigma-Aldrich

Aggrecan from bovine articular cartilage

lyophilized powder

Synonyme(s) :

Aggrecan Protein

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About This Item

Numéro MDL:
MFCD00676441
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.75

Source biologique

bovine articular cartilage

Niveau de qualité

200

Forme

lyophilized powder

Conditionnement

glass bottle of 1 mg

Technique(s)

cell culture | mammalian: suitable

Impuretés

salt, essentially free

Couleur

white

Solubilité

H2O: 2 mg/mL

Numéro d'accès UniProt

P13608

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

cow ... AGC1(280985)

Application

Aggrecan is a critical component for cartilage structure and the function of joints. Its synthesis and degradation are being studied for their roles in cartilage deterioration during joint injury disease and aging.

Actions biochimiques/physiologiques

This molecule produces a rigid, reversibly deformable gel that resists compression. It combines with hyaluronic acid to form very large macromolecular complexes that have an increased hydrohynamic volume and a significant increase (30-40%) in the relative viscosity of the solution.

Composants

Aggrecan is a major structural proteoglycan of the cartilage extracellular matrix. It is a large proteoglycan, with a molecular weight greater than 2,500 kDa composed of approximately 100-150 glycosaminoglycan chains attached to a core protein. The majority of these glycosaminoglycan chains are chondroitin/dermatan sulfate. Aggrecan contains three globular domains, G1, G2, and G3, which are involved in aggregation and hylauronan binding, cell adhesion and chondrycte apoptosis.

Attention

Store this product at -20°C. Stored as supplied, this powder shows little decomposition in 3 years when stored properly.

Notes préparatoires

This product is extracted from articular cartilage, chematographically purified, dialyzed against water, and 0.2 μm filtered prior to lyophilization. Once lyophilized, this powder is essentially salt-free. The product is soluble in water at 2 mg/mL.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Doege, K.J. et al.
Guidebook to the Extracellular Matrix and Adhesion Proteins, 17-18 (1993)
H Hedlund et al.
The Journal of biological chemistry, 274(9), 5777-5781 (1999-02-20)
Aggrecan, the predominant large proteoglycan of cartilage, is a multidomain macromolecule with each domain contributing specific functional properties. One of the domains contains the majority of the keratan sulfate (KS) chain substituents and a protein segment with a proline-rich hexapeptide
C J Billington et al.
The Biochemical journal, 336 ( Pt 1), 207-212 (1998-11-10)
The breakdown of aggrecan in cartilage is, in part, mediated by an enzyme named aggrecanase that cleaves within the interglobular domain of the molecule between a glutamic residue and an alanine residue. Although the enzyme cleavage site has been identified
L Cao et al.
Matrix biology : journal of the International Society for Matrix Biology, 17(5), 379-392 (1998-11-20)
The proteoglycan aggrecan is a major component of cartilage, and degradation of aggrecan is associated with aging and a number of pathological conditions. To investigate the effects of the accumulation of G1 domain from degraded aggrecan, we overexpressed the G1
M C Bolton et al.
The Biochemical journal, 337 ( Pt 1), 77-82 (1998-12-17)
The rates of incorporation of radiolabelled leucine into aggrecan and link protein have been measured in human articular cartilage of different ages. Aggrecan and link protein were purified in the A1 fraction of CsCl gradients as a result of their
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Articles

Glycosaminoglycans and Proteoglycans

There are five identified glycosaminoglycan chains (see Figure 1): Hyaluronan is not sulfated, but the other glycosaminoglycan chains contain sulfate substituents at various positions of the chain.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

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