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68957

Sigma-Aldrich

Tris(2-carboxyéthyl)phosphine hydrochloride

BioUltra, suitable for electrophoresis, SDS-PAGE tested

Synonyme(s) :

TCEP

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About This Item

Formule empirique (notation de Hill):
C9H15O6P · HCl
Numéro CAS:
51805-45-9
Poids moléculaire :
286.65
Numéro Beilstein :
3724376
Numéro MDL:
MFCD00145469
Code UNSPSC :
12352128
ID de substance PubChem :
329761275
Nomenclature NACRES :
NA.32

Gamme de produits

BioUltra

Pureté

97.5-102.5%

Forme

powder

Technique(s)

electrophoresis: suitable

Solubilité

H2O: soluble

Adéquation

SDS-PAGE tested

Chaîne SMILES 

Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O

InChI

1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H

Clé InChI

PBVAJRFEEOIAGW-UHFFFAOYSA-N

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Description générale

Tris(2-carboxyethyl) phosphine hydrochloride (TCEP.HCL ) is a useful reducing agent for Sulphur and nitrogen-containing compounds. TCEP.HCL and the resultant oxide are water-soluble. TCEP.HCL has electron-rich phosphorus and oxygen atoms to combine with various metals, including Rh, Ir, and Pd. It rapidly complexes with Rh and Ir in dioxane at ambient temperature. It reduces disulfide linkages in a range of peptides and proteins in biological disulfide.

Application

TCEP.HCL has been used in various bioconjugation applications, both in vitro and in vivo, to tag proteins and live organisms with great effectiveness under mild physiological circumstances. TCEP.HCL is suitable for varied biological and synthetic applications. At an acidic pH, TCEP.HCL can be used to convert organic disulfides to thiols in water irreversibly.
Water soluble reagent, used for selective reduction of disulfides. More stable than DTT and useful in mass spectrometry applications.

Caractéristiques et avantages

The product has the following benefits:

  • TCEP.HCL lowers reactive oxygen species levels.
  • It increases glutathione levels and mitochondrial content.
  • It has anti-cancer effects.

Remarque sur l'analyse

agarose gel electrophorese corresponds to requirements

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H314

Classification des risques

Eye Dam. 1 - Skin Corr. 1B

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Siming Chen et al.
Chemical communications (Cambridge, England), 49(12), 1226-1228 (2013-01-05)
A widely used reducing agent tris-(2-carboxyethyl) phosphine significantly promotes the reaction of cisplatin with Sp1 zinc finger protein. This discovery clarifies the reactivity of cisplatin towards Sp1 zinc finger protein and implies that the reactions of platinum drugs could be
Fang-Fang Cheng et al.
The Analyst, 139(16), 3860-3865 (2014-07-01)
The expression of microRNAs (miRNAs) is related to some cancer diseases. Recently, miRNAs have emerged as new candidate diagnostic and prognostic biomarkers for detecting a wide variety of cancers. Due to low levels, short sequences and high sequence homology among
Yan Li et al.
Biosensors & bioelectronics, 67, 364-369 (2014-09-10)
The development of sensitive telomerase biosensors is hindered by the restricted accessibility of telomere strand (TS) primer and the limited enzyme reaction space, which is mainly confined by the vertical distance. In this work, we designed an electrochemical telomerase biosensor
K Zarschler et al.
Nanoscale, 6(11), 6046-6056 (2014-04-30)
For effective localization of functionalized nanoparticles at diseased tissues such as solid tumours or metastases through biorecognition, appropriate targeting vectors directed against selected tumour biomarkers are a key prerequisite. The diversity of such vector molecules ranges from proteins, including antibodies
Nicola J Blackmore et al.
The Journal of biological chemistry, 290(29), 18187-18198 (2015-06-03)
Allostery, where remote ligand binding alters protein function, is essential for the control of metabolism. Here, we have identified a highly sophisticated allosteric response that allows complex control of the pathway for aromatic amino acid biosynthesis in the pathogen Mycobacterium
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